Isolation and characterization of new microsatellite markers in mango (Mangifera indica)

被引：41

作者：

Honsho, C

Nishiyama, K

Eiadthong, W

Yonemori, K ^{[1
]}

机构：

[1] Kyoto Univ, Grad Sch Agr, Sakyo Ku, Kyoto 6068502, Japan

[2] Kasetsart Univ, Fac Forestry, Bangkok 10900, Thailand

[3] Miyazaki Univ, Fac Agr, Miyazaki 8892192, Japan

[4] Okayama Univ, Fac Agr, Lab Postharvest Agr, Tsushima, Okayama 7008530, Japan

来源：

MOLECULAR ECOLOGY NOTES | 2005年 / 5卷 / 01期

关键词：

cultivar identification; enriched library; mango; microsatellite;

D O I：

10.1111/j.1471-8286.2004.00866.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Six microsatellite loci that were isolated from a microsatellite-enriched genomic library of mango (Mangifera indica) along with their specific primer sets were each characterized by using 36 cultivars collected mainly in Thailand. The observed and expected heterozygosities ranged from 0 to 0.83 and from 0.29 to 0.73, respectively. The number of putative alleles are two to six. Three of the six alleles have frequencies of over 75%. The high frequency may be attributed to the bias in the origin of cultivars. Among 36 mango cultivars tested, 29 cultivars showed a unique pattern by six primer sets, whereas seven cultivars cannot be identified because of genotype similarities. This suggests the potentials for identification of mango cultivars by microsatellite markers.

引用

页码：152 / 154

页数：3

共 8 条

[1] Identification of mango cultivars of Thailand and evaluation of their genetic variation using the amplified fragments by simple sequence repeat-(SSR-) anchored primers [J].