Use of locked nucleic acid oligonucleotides as hybridization/FRET probes for quantification of 16S rDNA by real-time PCR

被引：11

作者：

Goldenberg, O

Landt, O

Schumann, RR

Göbel, UB

Hamann, L

机构：

[1] TIB MOLBIOL, Berlin, Germany

[2] Charite Univ Med Ctr, Berlin, Germany

来源：

BIOTECHNIQUES | 2005年 / 38卷 / 01期

关键词：

D O I：

10.2144/05381BM01

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

引用

页码：29 / +

页数：3

共 16 条

[1] Review and re-analysis of domain-specific 16S primers [J].

Baker, GC ;

Smith, JJ ;

Cowan, DA .

JOURNAL OF MICROBIOLOGICAL METHODS, 2003, 55 (03) :541-555

[2]

BORIELLO SP, 1986, MICROBIAL METABOLISM, P2

[3] Gut flora in health and disease [J].

Guarner, F ;

Malagelada, JR .

LANCET, 2003, 361 (9356) :512-519

[4] Locked nucleic acid (LNA) single nucleotide polymorphism (SNP) genotype analysis and validation using real-time PCR [J].

Johnson, MP ;

Haupt, LM ;

Griffiths, LR .

NUCLEIC ACIDS RESEARCH, 2004, 32 (06) :e55

[5]

Kalliomäki M, 2003, CURR OPIN ALLERGY CL, V3, P15, DOI [10.1097/00130832-200302000-00003, 10.1097/01.all.0000053262.39029.a1]

[6] LNA (Locked Nucleic Acids): Synthesis of the adenine, cytosine, guanine, 5-methylcytosine, thymine and uracil bicyclonucleoside monomers, oligomerisation, and unprecedented nucleic acid recognition [J].

Koshkin, AA ;

Singh, SK ;

Nielsen, P ;

Rajwanshi, VK ;

Kumar, R ;

Meldgaard, M ;

Olsen, CE ;

Wengel, J .

TETRAHEDRON, 1998, 54 (14) :3607-3630

[7] Evaluation of the performance of LNA and MGB probes in 5′-nuclease PCR assays [J].

Letertre, C ;

Perelle, S ;

Dilasser, F ;

Arar, K ;

Fach, P .

MOLECULAR AND CELLULAR PROBES, 2003, 17 (06) :307-311

[8] Quantification of intestinal bacterial populations by real-time PCR with a universal primer set and minor groove binder probes: a global approach to the enteric flora [J].

Ott, SJ ;

Musfeldt, M ;

Ullmann, U ;

Hampe, J ;

Schreiber, S .

JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (06) :2566-2572

[9] Alterations of the dominant faecal bacterial groups in patients with Crohn's disease of the colon [J].

Seksik, P ;

Rigottier-Gois, L ;

Gramet, G ;

Sutren, M ;

Pochart, P ;

Marteau, P ;

Jian, R ;

Doré, J .

GUT, 2003, 52 (02) :237-242

[10] FISHing with locked nucleic acids (LNA): evaluation of different LNA/DNA mixmers [J].

Silahtaroglu, AN ;

Tommerup, N ;

Vissing, H .

MOLECULAR AND CELLULAR PROBES, 2003, 17 (04) :165-169

← 1 2 →