PA1b, a plant peptide, induces intracellular [Ca2+] increase via Ca2+ influx through the L-type Ca2+ channel and triggers secretion in pancreatic β cells

Using alginic acid to adsorb polypeptides at pH 2.7, we isolated a peptide pea albumin lb (PA1b) from pea seeds. The PA1b is a single chain peptide consisting of 37 amino acid residues with 6 cysteines which constitutes the cystine-knot structure. Using microfluorometry and patch clamp techniques, we found that PA1b significantly elevated the intracellular calcium level ([Ca2+](i)) and elicited membrane capacitance increase in the primary rat pancreatic beta cells. The PA1b effect on [Ca2+](i) elevation was abolished in the absence of extracellular Ca2+ or in the presence of L-type Ca 2+ channel blocker, nimodipine. Interestingly, ive found that PA1b significantly depolarized membrane potential, which could lead to the opening of voltage-dependent L-type Ca2+, channels and influx of extracellular Ca2+, and then evoke robust secretion. In this study we identified the plant peptide PA1b which is capable of affecting the excitability and function of mammalian pancreatic beta cell.