Membrane-SPINE: An improved method to identify protein-protein interaction partners of membrane proteins in vivo

被引：16

作者：

Mueller, Volker S. ^{[1
]}

Jungblut, Peter R. ^{[2
]}

Meyer, Thomas F. ^{[3
]}

Hunke, Sabine ^{[1
]}

机构：

[1] Humboldt Univ, Dept Biol, D-10115 Berlin, Germany

[2] Max Planck Inst Infect Biol, Core Facil Prot Anal, Berlin, Germany

[3] Max Planck Inst Infect Biol, Dept Mol Biol, Berlin, Germany

来源：

PROTEOMICS | 2011年 / 11卷 / 10期

关键词：

Membrane proteins; Membrane-Strep-tagged protein interaction experiment; MS-analysis; Protein-protein interaction; Salmonella enterica serovar Typhimurium; Technology; ENVELOPE STRESS-RESPONSE; CROSS-LINKING; 2-COMPONENT; IDENTIFICATION; PURIFICATION; SYSTEMS;

D O I：

10.1002/pmic.201000558

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Membrane proteins are crucial for many essential cellular processes. As membrane proteins function in complexes, methods to detect and to characterize membrane protein-protein interactions are undoubtedly required. Therefore, we developed the "Membrane-Strep-tagged protein interaction experiment" (Membrane-SPINE) that combines the specific purification of a Strep-tagged membrane protein with the reversible fixation of protein complexes by formaldehyde cross-linking. In combination with MS analysis, we suggest Membrane-SPINE as a powerful tool to identify unknown interaction partners of membrane proteins in vivo.

引用

收藏

页码：2124 / 2128

页数：5

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