Genetically Encoded Immunophotosensitizer

被引：3
作者：
Serebrovskaya, E. O. [1 ]
Stremovsky, O. A. [1 ]
Chudakov, D. M. [1 ]
Lukyanov, K. A. [1 ]
Deyev, S. M. [1 ]
机构：
[1] Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow 117997, Russia
来源：
RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY | 2011年 / 37卷 / 01期
基金：
俄罗斯基础研究基金会;
关键词：
photoimmunotherapy; photosensitizer; fluorescent protein; miniantibody; HER2/neu; MONOCLONAL-ANTIBODIES; PHOTODYNAMIC THERAPY; FLUORESCENT PROTEIN; CANCER-CELLS; PHOTOSENSITIZER; CHROMOPHORE; STABILITY; CATALYSIS; OXIDATION;
D O I：
10.1134/S1068162011010134
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Conjugates of antibodies with photosensitizers are successfully used for the targeted killing of cancer cells bearing particular surface markers by the method known as photoimmunotherapy. However, the chemical conjugation of photosensitizers with antibodies poses a number of problems. Among these are a low reproducibility, aggregation, and the presence of impurities of the unconjugated photosensitizer. Here we describe a method of designing a fully genetically encoded immunophotosensitizer, which consists of the anti-HER2/neu miniantibody 4D5scFv as a targeting molecule and the phototoxic fluorescent protein KillerRed as a photosensitizing molecule. Both domains in the recombinant protein retained their functional properties: a high affinity for the HER2/neu antigen and phototoxicity. The recombinant protein 4D5scFv-KillerRed showed a high specificity for HER2/neu-overexpressing cancer cells and effectively lowered their viability upon irradiation.
引用
收藏
页码：123 / 129
页数：7
相关论文
共 31 条
  • [1] THE EFFECTS OF AGGREGATION, PROTEIN-BINDING AND CELLULAR INCORPORATION ON THE PHOTOPHYSICAL PROPERTIES OF BENZOPORPHYRIN DERIVATIVE MONOACID RING-A (BPDMA)
    AVELINE, BM
    HASAN, T
    REDMOND, RW
    [J]. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 1995, 30 (2-3) : 161 - 169
  • [2] DOWN-REGULATION OF THE EPIDERMAL GROWTH-FACTOR RECEPTOR IN KB CELLS IS DUE TO RECEPTOR INTERNALIZATION AND SUBSEQUENT DEGRADATION IN LYSOSOMES
    BEGUINOT, L
    LYALL, RM
    WILLINGHAM, MC
    PASTAN, I
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (08): : 2384 - 2388
  • [3] Chromophore-assisted light inactivation (CALI) using the phototoxic fluorescent protein KillerRed
    Bulina, Maria E.
    Lukyanov, Konstantin A.
    Britanova, Olga V.
    Onichtchouk, Daria
    Lukyanov, Sergey
    Chudakov, Dmitriy M.
    [J]. NATURE PROTOCOLS, 2006, 1 (02) : 947 - 953
  • [4] A genetically encoded photosensitizer
    Bulina, ME
    Chudakov, DM
    Britanova, OV
    Yanushevich, YG
    Staroverov, DB
    Chepurnykh, TV
    Merzlyak, EM
    Shkrob, MA
    Lukyanov, S
    Lukyanov, KA
    [J]. NATURE BIOTECHNOLOGY, 2006, 24 (01) : 95 - 99
  • [5] Photodynamic therapy: update 2006 - Part 2: Clinical results
    Calzavara-Pinton, P. G.
    Venturini, M.
    Sala, R.
    [J]. JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY, 2007, 21 (04) : 439 - 451
  • [6] Engineering antibodies for imaging and therapy
    Carter, P
    Merchant, AM
    [J]. CURRENT OPINION IN BIOTECHNOLOGY, 1997, 8 (04) : 449 - 454
  • [7] Mechanism for antibody catalysis of the oxidation of water by singlet dioxygen
    Datta, D
    Vaidehi, N
    Xu, X
    Goddard, WA
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (05) : 2636 - 2641
  • [8] Del Governatore M, 2000, BRIT J CANCER, V82, P56
  • [9] Modern Technologies for Creating Synthetic Antibodies for Clinical Application
    Deyev, S. M.
    Lebedenko, E. N.
    [J]. ACTA NATURAE, 2009, 1 (01): : 32 - 50
  • [10] Multivalency: the hallmark of antibodies used for optimization of tumor targeting by design
    Deyev, Sergey M.
    Lebedenko, Ekaterina N.
    [J]. BIOESSAYS, 2008, 30 (09) : 904 - 918
1234