Expression of Pseudomonas amyloderamosa isoamylase gene in Saccharomyces cerevisiae

被引：11

作者：

Chen, PH

Lin, LL

Hsu, WH ^{[1
]}

机构：

[1] Natl Chung Hsing Univ, Inst Mol Biol, Taichung 402, Taiwan

[2] Hung Kuang Inst Technol, Dept Food Sci & Nutr, Taichung, Taiwan

来源：

BIOTECHNOLOGY LETTERS | 1998年 / 20卷 / 08期

关键词：

D O I：

10.1023/A:1005382602683

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Isoamylase gene (iso) of Pseudomonas amyloderamosa was amplified by polymerase chain reaction and cloned into Saccharomyces cerevisiae vectors under the control of alcohol dehydrogenase gene and glyceraldehyde-3-phosphate dehydrogenase gene promoters. The signal sequence of iso gene was also replaced with that of Schwanniomyces occidentalis alpha-amylase gene. The extracellular isoamylase activity of transformed Sacc. cerevisiae could reach 86 U ml(-1) after a 4-days cultivation.

引用

页码：735 / 739

页数：5

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