Expression of Pseudomonas amyloderamosa isoamylase gene in Saccharomyces cerevisiae

被引：11
作者：
Chen, PH
Lin, LL
Hsu, WH [1 ]
机构：
[1] Natl Chung Hsing Univ, Inst Mol Biol, Taichung 402, Taiwan
[2] Hung Kuang Inst Technol, Dept Food Sci & Nutr, Taichung, Taiwan
来源：
BIOTECHNOLOGY LETTERS | 1998年 / 20卷 / 08期
关键词：
D O I：
10.1023/A:1005382602683
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Isoamylase gene (iso) of Pseudomonas amyloderamosa was amplified by polymerase chain reaction and cloned into Saccharomyces cerevisiae vectors under the control of alcohol dehydrogenase gene and glyceraldehyde-3-phosphate dehydrogenase gene promoters. The signal sequence of iso gene was also replaced with that of Schwanniomyces occidentalis alpha-amylase gene. The extracellular isoamylase activity of transformed Sacc. cerevisiae could reach 86 U ml(-1) after a 4-days cultivation.
引用
收藏
页码：735 / 739
页数：5
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