Proteomic Profiling of Aging in Glomeruli of Mice by using Two-Dimensional Differential Gel Electrophoresis

被引:0
作者
Liu, Xiaodan [1 ]
Fan, Qiuling [1 ]
Yang, Gang [1 ]
Wang, Lining [1 ]
机构
[1] China Med Univ, Affiliated Hosp 1, Dept Nephrol, Shenyang 110001, Liaoning, Peoples R China
来源
MEDICAL SCIENCE MONITOR | 2015年 / 21卷
基金
中国国家自然科学基金;
关键词
Aging; Kidney Glomerulus; Proteomics; Two-Dimensional Difference Gel Electrophoresis; ATP SYNTHASE; KIDNEY; DETERMINANTS; EXPRESSION;
D O I
暂无
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Background: Glomerular proteins were analyzed by proteomics to screen proteins participating in maturation of glomeruli before senescence and to find key proteins involved in the aging process. Material/Methods: Glomeruli of C57BL/6 mice at 8 and 20 weeks were separated by kidney perfusion. Proteomic profiles of glomeruli were investigated by using two-dimensional differential gel electrophoresis and MALDI-TOF mass spectrometry. Results: We identified 22 differentially expressed proteins. Among them, 3 proteins were significantly up-regulated and 19 proteins were significantly down-regulated in mature mice. Out of these 22 proteins, 18% take part in protein transport, protein targeting, and proteolysis; 5% in glycolysis; 14% in transcription; 9% in electron transport; 9% were chaperones; and 9% were hydrolases. Conclusions: Our results provide insights into the glomerular differentially expressed proteins correlated with renal aging. In this study we found that aging altered the expression of ATP synthase subunit beta. Further studies on this protein might help to understand the mechanism of renal aging.
引用
收藏
页码:419 / 425
页数:7
相关论文
共 28 条
[1]   Autoantibodies to Endothelial Cell Surface ATP Synthase, the Endogenous Receptor for Hsp60, Might Play a Pathogenic Role in Vasculatides [J].
Alard, Jean-Eric ;
Hillion, Sophie ;
Guillevin, Loic ;
Saraux, Alain ;
Pers, Jacques-Olivier ;
Youinou, Pierre ;
Jamin, Christophe .
PLOS ONE, 2011, 6 (02)
[2]   Proteomic study on gender differences in aging kidney of mice [J].
Amelina, Hanna ;
Cristobal, Susana .
PROTEOME SCIENCE, 2009, 7
[3]   Changes in renal hemodynamics and structure in the aging kidney; sexual dimorphism and the nitric oxide system [J].
Baylis, C .
EXPERIMENTAL GERONTOLOGY, 2005, 40 (04) :271-278
[4]   The aging kidney revisited: A systematic review [J].
Bolignano, Davide ;
Mattace-Raso, Francesco ;
Sijbrands, Eric J. G. ;
Zoccali, Carmine .
AGEING RESEARCH REVIEWS, 2014, 14 :65-80
[5]   ATP synthase is responsible for maintaining mitochondrial membrane potential in bloodstream form Trypanosoma brucei [J].
Brown, SV ;
Hosking, P ;
Li, JL ;
Williams, N .
EUKARYOTIC CELL, 2006, 5 (01) :45-53
[6]   Proteome profiling of aging in mouse models: Differential expression of proteins involved in metabolism, transport, and stress response in kidney [J].
Chakravarti, Bulbul ;
Seshi, Beerelli ;
Ratanaprayul, Wongrat ;
Dalal, Neville ;
Lin, Lawrence ;
Raval, Alpan ;
Chakravarti, Deb N. .
PROTEOMICS, 2009, 9 (03) :580-597
[7]   AGE-RELATED GLOMERULAR SCLEROSIS - BASE-LINE VALUES IN HONG-KONG [J].
CHAN, KW ;
LEUNG, CY ;
CHAN, CW .
PATHOLOGY, 1990, 22 (04) :177-180
[8]   The metabolite α-ketoglutarate extends lifespan by inhibiting ATP synthase and TOR [J].
Chin, Randall M. ;
Fu, Xudong ;
Pai, Melody Y. ;
Vergnes, Laurent ;
Hwang, Heejun ;
Deng, Gang ;
Diep, Simon ;
Lomenick, Brett ;
Meli, Vijaykumar S. ;
Monsalve, Gabriela C. ;
Hu, Eileen ;
Whelan, Stephen A. ;
Wang, Jennifer X. ;
Jung, Gwanghyun ;
Solis, Gregory M. ;
Fazlollahi, Farbod ;
Kaweeteerawat, Chitrada ;
Quach, Austin ;
Nili, Mahta ;
Krall, Abby S. ;
Godwin, Hilary A. ;
Chang, Helena R. ;
Faull, Kym F. ;
Guo, Feng ;
Jiang, Meisheng ;
Trauger, Sunia A. ;
Saghatelian, Alan ;
Braas, Daniel ;
Christofk, Heather R. ;
Clarke, Catherine F. ;
Teitell, Michael A. ;
Petrascheck, Michael ;
Reue, Karen ;
Jung, Michael E. ;
Frand, Alison R. ;
Huang, Jing .
NATURE, 2014, 510 (7505) :397-401
[9]  
Epstein M, 1996, J AM SOC NEPHROL, V7, P1106
[10]   Marked interindividual variability in renal maturation of preterm infants: lessons from autopsy [J].
Faa, Gavino ;
Gerosa, Clara ;
Fanni, Daniela ;
Nemolato, Sonia ;
Locci, Annalisa ;
Cabras, Tiziana ;
Marinelli, Viviana ;
Puddu, Melania ;
Zaffanello, Marco ;
Monga, Guido ;
Fanos, Vassilios .
JOURNAL OF MATERNAL-FETAL & NEONATAL MEDICINE, 2010, 23 :129-133