On-chip single cell lysis integrated with micro-flow cytometry

被引：0

作者：

Poulsen, CR ^{[1
]}

Ramsey, JM ^{[1
]}

机构：

[1] Univ N Carolina, Dept Chem, Chapel Hill, NC 27599 USA

来源：

Micro Total Analysis Systems 2004, Vol 2 | 2005年 / 297期

关键词：

cell lysis; flow cytometry; signaling pathways; single cell assays;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We have developed a microfluidic system that integrates flow cytometry, single cell lysis, and separation of cellular components. Hydrodynamic flow is used to drive the fluids through the system. An electrical field orthogonal to the cell flow automatically lyses the cells and injects the lysate into a separation channel for detection. Fluorescently labeled Jurkat cells were used to demonstrate the systems capability of continuous and simultaneous flow cytometry and single cell lysis.

引用

页码：162 / 164

页数：3

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