Structure and inhibition of EV-D68, a virus that causes respiratory illness in children

被引：140

作者：

Liu, Yue ^{[1
]}

Sheng, Ju ^{[1
]}

Fokine, Andrei ^{[1
]}

Meng, Geng ^{[1
]}

Shin, Woong-Hee ^{[1
]}

Long, Feng ^{[1
]}

Kuhn, Richard J. ^{[1
]}

Kihara, Daisuke ^{[1
,2
]}

Rossmann, Michael G. ^{[1
]}

机构：

[1] Purdue Univ, Dept Biol Sci, W Lafayette, IN 47907 USA

[2] Purdue Univ, Dept Comp Sci, W Lafayette, IN 47907 USA

来源：

SCIENCE | 2015年 / 347卷 / 6217期

基金：

美国国家卫生研究院;

关键词：

HUMAN RHINOVIRUS-14; ENTEROVIRUS; 71; PICORNAVIRUS; PLECONARIL; POLIOVIRUS; RESOLUTION; SUSCEPTIBILITY; ATTACHMENT; SEROTYPES; FEATURES;

D O I：

10.1126/science.1261962

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Enterovirus D68 (EV-D68) is a member of Picornaviridae and is a causative agent of recent outbreaks of respiratory illness in children in the United States. We report here the crystal structures of EV-D68 and its complex with pleconaril, a capsid-binding compound that had been developed as an anti-rhinovirus drug. The hydrophobic drug-binding pocket in viral protein 1 contained density that is consistent with a fatty acid of about 10 carbon atoms. This density could be displaced by pleconaril. We also showed that pleconaril inhibits EV-D68 at a half-maximal effective concentration of 430 nanomolar and might, therefore, be a possible drug candidate to alleviate EV-D68 outbreaks.

引用

页码：71 / 74

页数：4

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