Surface-enhanced Raman scattering imaging of single living lymphocytes with multivariate evaluation

被引：44
作者：
Eliasson, C
Lorén, A
Engelbrektsson, J
Josefson, M
Abrahamsson, J
Abrahamsson, K
机构：
[1] Chalmers Univ Technol, Dept Chem & Biosci Analyt & Marine Chem, SE-41296 Gothenburg, Sweden
[2] AstraZeneca, Pharmaceut Analyt R&D, SE-43183 Molndal, Sweden
[3] Sahlgrens Univ Hosp, Dept Paediat, SE-41685 Gothenburg, Sweden
来源：
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2005年 / 61卷 / 04期
关键词：
surface-enhanced Raman scattering; SERS; imaging; single cells; lymphocyte; rhodamine; 6G; principal component analysis; PCA; multivariate analysis;
D O I：
10.1016/j.saa.2004.05.038
中图分类号：
O433 [光谱学];
学科分类号：
0703 ; 070302 ;
摘要：
This paper is aimed to show the possibility to determine individual organic compounds introduced into single living cells with surface-enhanced Raman spectroscopy (SERS). Surface enhancement was achieved with gold colloids that were allowed to diffuse into lymphocytes. An introduced analyte, rhodamine 6G, could be imaged together with for example nucleotides and amino acids of the cell. Multivariate evaluation of surface-enhanced Raman images proved to be a powerful tool for the separation of spectral information of various intracellular components. The principal component analysis (PCA) enabled identification of spectra containing different chemical information and separation of the spectral contribution of rhodamine 6G from the complex cellular matrix. (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码：755 / 760
页数：6
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