Characterizing the Structure and Interactions of Model Lipid Membranes Using Electrophysiology

被引:15
作者
El-Beyrouthy, Joyce [1 ]
Freeman, Eric [1 ]
机构
[1] Univ Georgia, Coll Engn, Sch Environm Civil Agr & Mech Engn, Athens, GA 30602 USA
基金
美国国家科学基金会;
关键词
model membranes; electrophysiology; membrane-particle interactions; lipid bilayer electrostatics; conductive channels; soft capacitor; impedance analysis; DROPLET-INTERFACE BILAYERS; SINGLE-CHANNEL RECORDINGS; FLUID MOSAIC MODEL; IMPEDANCE SPECTROSCOPY; ANTIMICROBIAL PEPTIDES; MOLECULAR-ORGANIZATION; PHOSPHOLIPID-MEMBRANES; DIELECTRIC STRUCTURE; SUPPORTED MEMBRANES; WATER PERMEABILITY;
D O I
10.3390/membranes11050319
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The cell membrane is a protective barrier whose configuration determines the exchange both between intracellular and extracellular regions and within the cell itself. Consequently, characterizing membrane properties and interactions is essential for advancements in topics such as limiting nanoparticle cytotoxicity. Characterization is often accomplished by recreating model membranes that approximate the structure of cellular membranes in a controlled environment, formed using self-assembly principles. The selected method for membrane creation influences the properties of the membrane assembly, including their response to electric fields used for characterizing transmembrane exchanges. When these self-assembled model membranes are combined with electrophysiology, it is possible to exploit their non-physiological mechanics to enable additional measurements of membrane interactions and phenomena. This review describes several common model membranes including liposomes, pore-spanning membranes, solid supported membranes, and emulsion-based membranes, emphasizing their varying structure due to the selected mode of production. Next, electrophysiology techniques that exploit these structures are discussed, including conductance measurements, electrowetting and electrocompression analysis, and electroimpedance spectroscopy. The focus of this review is linking each membrane assembly technique to the properties of the resulting membrane, discussing how these properties enable alternative electrophysiological approaches to measuring membrane characteristics and interactions.
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页数:30
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