Determination of mitomycin C in urinary bladder tissue by reversed-phase high-performance liquid chromatography

被引：1

作者：

Sidorova, A. A. ^{[1
]}

Kartsova, L. A. ^{[2
]}

Grigoriev, A. V. ^{[1
]}

Protoschak, V. V. ^{[3
]}

Murashko, E. A. ^{[2
]}

机构：

[1] CSU Analyt Spectrometry, St Petersburg 195220, Russia

[2] St Petersburg State Univ, Fac Chem, St Petersburg 198504, Russia

[3] SM Kirov Mil Med Acad, St Petersburg 190013, Russia

来源：

JOURNAL OF ANALYTICAL CHEMISTRY | 2010年 / 65卷 / 08期

关键词：

CANCER; DRUGS; WALL;

D O I：

10.1134/S1061934810080137

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A procedure was developed for the determination of an anticancer drug of mitomycin C (MMC) in urinary bladder tissue samples by reversed-phase high-performance liquid chromatography with spectrophotometric (365 nm) and mass-spectrometric detection with detection limits of 50 and 10 ng/mL, respectively (signal-to-noise ratio = 3). The tissue samples were homogenized and solid-phase extraction was performed on a DSC-18 cartridge. The procedure was used for comparing the effectiveness of the MMC penetration into the urinary bladder tissue by passive diffusion and by intravesical drug electrophoresis. EDMA increases the rate of mitomycin penetration into urinary bladder walls by 3 to 5 times with respect to passive diffusion.

引用

页码：838 / 842

页数：5

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