The G2/M checkpoint phosphatase cdc25C is located within centrosomes

被引：13

作者：

Busch, Corinna ^{[1
]}

Barton, Olivia ^{[1
]}

Morgenstern, Eberhard ^{[1
]}

Goetz, Claudia ^{[1
]}

Guenther, Juergen ^{[1
]}

Noll, Andreas ^{[1
]}

Montenarh, Mathias ^{[1
]}

机构：

[1] Univ Saarland, D-66424 Homburg, Germany

来源：

INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY | 2007年 / 39卷 / 09期

关键词：

centrosomes; cdc25C; phosphatase; CENP-F; mitosis;

D O I：

10.1016/j.biocel.2007.04.022

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

cdc25C is a phosphatase which regulates the activity of the mitosis promoting factor cyclin B/cdk1 by dephosphorylation, thus triggering G(2)/M transition. The activity and the sub-cellular localisation of cdc25C are regulated by phosphorylation. It is well accepted that cdc25C has to enter the nucleus to activate the cyclin B/cdkI complex at G(2)/M transition. Here, we will show that cdc25C is located in the cytoplasm at defined dense structures, which according to immunofluorescence analysis, electron microscopy as well as biochemical subfractionation, are proven to be the centrosomes. Since cyclin B and cdk1 are also located at the centrosomes, this subfraction of cdc25C might participate in the control of the onset of mitosis suggesting a further role for cdc25C at the centrosomes. (c) 2007 Elsevier Ltd. All rights reserved.

引用

页码：1707 / 1713

页数：7

共 35 条

[1] Checkpoint kinase 2 (Chk2) monomers or dimers phosphorylate Cdc25C after DNA damage regardless of threonine 68 phosphorylation [J].