Leukemia inhibitory factor enhances mast cell growth in a mast cell/fibroblast co-culture system through Stat3 signaling pathway of fibroblasts

被引：10

作者：

Hiragun, T ^{[1
]}

Morita, E ^{[1
]}

Mihara, S ^{[1
]}

Tanaka, T ^{[1
]}

Gyotoku, E ^{[1
]}

Kameyoshi, Y ^{[1
]}

Yamamoto, S ^{[1
]}

机构：

[1] Hiroshima Univ, Sch Med, Dept Dermatol, Hiroshima, Japan

来源：

FEBS LETTERS | 2000年 / 487卷 / 02期

关键词：

bone marrow-derived mast cell; co-culture; leukemia inhibitory factor; signal transducer and activator of transcription 3; 3T3; fibroblast;

D O I：

10.1016/S0014-5793(00)02355-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Leukemia inhibitory factor (LIF) enhanced mast cell growth in a mast cell/3T3 fibroblast co-culture system, however the precise mechanisms have not been defined. Western blot analysis showed that bone marrow-derived mast cells failed to express both LIF receptor (LIFR) and gp130, whereas 3T3 fibroblasts expressed both LIFR and gp130. This result indicates that the activity of LIF for mast cell growth is mediated by 3T3 fibroblasts. Signal transducer and activator of transcription (Stat) 3-transfected 3T3 fibroblasts enhanced mast cell growth. In addition, dominant-negative Stat3-transfected fibroblasts blocked LIE-mediated mast cell growth in the co-culture system. In conclusion, LIF-induced mast cell growth in the co-culture system is mediated by an indirect pathway via 3T3 fibroblasts through activating Stat3 signaling pathway in 3T3 fibroblasts. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：219 / 223

页数：5

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