Urinary extracellular vesicles: A position paper by the Urine Task Force of the International Society for Extracellular Vesicles

被引：259

作者：

Erdbrugger, Uta ^{[1
]}

Blijdorp, Charles J. ^{[2
]}

Bijnsdorp, Irene V. ^{[6
]}

Borras, Francesc E. ^{[7
,8
]}

Burger, Dylan ^{[9
,10
]}

Bussolati, Benedetta ^{[11
]}

Byrd, James Brian ^{[12
]}

Clayton, Aled ^{[13
]}

Dear, James W. ^{[14
]}

Falcon-Perez, Juan M. ^{[15
]}

Grange, Cristina ^{[16
]}

Hill, Andrew F. ^{[17
]}

Holthofer, Harry ^{[18
,19
]}

Hoorn, Ewout J. ^{[2
]}

Jenster, Guido ^{[5
]}

Jimenez, Connie R. ^{[6
]}

Junker, Kerstin ^{[20
]}

Klein, John ^{[21
]}

Knepper, Mark A. ^{[22
]}

Koritzinsky, Erik H. ^{[23
]}

Luther, James M. ^{[24
]}

Lenassi, Metka ^{[25
]}

Leivo, Janne ^{[26
]}

Mertens, Inge ^{[27
,28
]}

Musante, Luca ^{[1
]}

Oeyen, Eline ^{[27
,28
]}

Puhka, Maija ^{[19
]}

van Royen, Martin E. ^{[29
]}

Sanchez, Catherine ^{[30
]}

Soekmadji, Carolina ^{[31
]}

Thongboonkerd, Visith ^{[32
]}

van Steijn, Volkert ^{[33
]}

Verhaegh, Gerald ^{[34
]}

Webber, Jason P. ^{[35
]}

Witwer, Kenneth ^{[36
,37
]}

Yuen, Peter S. T. ^{[23
]}

Zheng, Lei ^{[38
]}

Llorente, Alicia ^{[3
,4
,6
]}

Martens-Uzunova, Elena S. ^{[5
]}

机构：

[1] Univ Virginia Hlth Syst, Div Nephrol, Charlottesville, VA USA

[2] Erasmus MC, Univ Med Ctr Rotterdam, Div Nephrol & Transplantat, Dept Internal Med, Rotterdam, Netherlands

[3] Oslo Univ Hosp, Inst Canc Res, Dept Mol Cell Biol, Oslo, Norway

[4] Oslo Metropolitan Univ, Dept Mech Elect & Chem Engn, Oslo, Norway

[5] Erasmus MC Canc Inst, Univ Med Ctr Rotterdam, Dept Urol, Lab Expt Urol, Rotterdam, Netherlands

[6] Amsterdam UMC locat VUmc, Canc Ctr Amsterdam, Dept Urol, Amsterdam, Netherlands

[7] Germans Trias & Pujol Univ Hosp, REMAR IVECAT Grp, Germans Trias Pujol Hlth Sci Res Inst, Badalona, Spain

[8] Germans Trias & Pujol Univ Hosp, Dept Nephrol, Badalona, Spain

[9] Univ Ottawa, Ottawa Hosp Res Inst, Kidney Res Ctr, Ottawa, ON, Canada

[10] Univ Ottawa, Dept Cellular & Mol Med, Ottawa, ON, Canada

[11] Univ Torino, Dept Mol Biotechnol & Hlth Sci, Turin, Italy

[12] Univ Michigan, Dept Internal Med, Sch Med, Ann Arbor, MI 48109 USA

[13] Cardiff Univ, Sch Med, Div Canc & Genet, Tissue Microenvironm Grp, Cardiff, Wales

[14] Univ Edinburgh, Queens Med Res Inst, Ctr Cardiovasc Sci, Edinburgh, Midlothian, Scotland

[15] CIC bioGUNEBRTA, Exosomes Lab & Metabol Platform, Bizkaia Technol Pk, Derio, Spain

[16] Univ Torino, Dept Med Sci, Turin, Italy

[17] La Trobe Univ, Trobe Inst Mol Sci, Dept Biochem & Genet, Bundoora, Vic, Australia

[18] Univ Clin Hamburg Eppendorf, Internal Med, Hamburg, Germany

[19] Univ Helsinki, Inst Mol Med Finland, FIMM, Helsinki, Finland

[20] Saarland Univ, Dept Urol, Med Ctr, Homburg, Germany

[21] Univ Louisville, Dept Med, Sch Med, Louisville, KY USA

[22] NHLBI, Epithelial Syst Biol Lab, Syst Biol Ctr, NIH, Bldg 10, Bethesda, MD 20892 USA

[23] NIDDK, Renal Diagnost & Therapeut Unit, NIH, Bethesda, MD USA

[24] Vanderbilt Univ, Med Ctr, Dept Med, Div Clin Pharmacol, 221 Kirkland Hall, Nashville, TN 37235 USA

[25] Univ Ljubljana, Inst Biochem, Fac Med, Ljubljana, Slovenia

[26] Univ Turku, Dept Biochem, Turku, Finland

[27] Univ Antwerp, Ctr Prote, Antwerp, Belgium

[28] VITO, Hlth Unit, Antwerp, Belgium

[29] Erasmus MC, Univ Med Ctr Rotterdam, Erasmus MC Canc Inst, Dept Pathol, Rotterdam, Netherlands

[30] Clin Ias Condes, Dept Urol, Res Core, Santiago, Chile

[31] QIMR Berghofer Med Res Inst, Dept Cell & Mol Biol, Brisbane, Qld, Australia

[32] Mahidol Univ, Fac Med Siriraj Hosp, Med Prote Unit, Off Res & Dev, Bangkok, Thailand

[33] Delft Univ Technol, Dept Chem Engn, Delft, Netherlands

[34] Radboud Univ Nijmegen Med Ctr, Dept Urol, Nijmegen, Netherlands

[35] Swansea Univ, Inst Life Sci, Med Sch, Singleton Pk Campus, Swansea, W Glam, Wales

[36] Johns Hopkins Univ, Sch Med, Dept Mol & Comparat Pathobiol, Baltimore, MD USA

[37] Johns Hopkins Univ, Sch Med, Dept Neurol, Baltimore, MD USA

[38] Southern Med Univ, Nanfang Hosp, Dept Lab Med, Guangzhou, Guangdong, Peoples R China

来源：

JOURNAL OF EXTRACELLULAR VESICLES | 2021年 / 10卷 / 07期

关键词：

biobank; biomarkers; bladder; extracellular vesicles; kidney; liquid biopsy; prostate; rigor and standardization; urine; SODIUM-CHLORIDE COTRANSPORTER; DEEP SEQUENCING ANALYSIS; TAMM-HORSFALL PROTEIN; PROSTATE-CANCER; PROTEOMIC ANALYSIS; MESSENGER-RNA; CLINICAL-APPLICATIONS; DIABETIC-NEPHROPATHY; BIOMARKER DISCOVERY; MEMBRANE-VESICLES;

D O I：

10.1002/jev2.12093

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Urine is commonly used for clinical diagnosis and biomedical research. The discovery of extracellular vesicles (EV) in urine opened a new fast-growing scientific field. In the last decade urinary extracellular vesicles (uEVs) were shown to mirror molecular processes as well as physiological and pathological conditions in kidney, urothelial and prostate tissue. Therefore, several methods to isolate and characterize uEVs have been developed. However, methodological aspects of EV separation and analysis, including normalization of results, need further optimization and standardization to foster scientific advances in uEV research and a subsequent successful translation into clinical practice. This position paper is written by the Urine Task Force of the Rigor and Standardization Subcommittee of ISEV consisting of nephrologists, urologists, cardiologists and biologists with active experience in uEV research. Our aim is to present the state of the art and identify challenges and gaps in current uEV-based analyses for clinical applications. Finally, recommendations for improved rigor, reproducibility and interoperability in uEV research are provided in order to facilitate advances in the field.

引用

页数：39

共 290 条

[1] Urinary Exosomal mRNA of WT1 as Diagnostic and Prognostic Biomarker for Diabetic Nephropathy [J].

Abe, Hideharu ;

Sakurai, Akiko ;

Ono, Hiroyuki ;

Hayashi, Sanae ;

Yoshimoto, Sakiya ;

Ochi, Arisa ;

Ueda, Sayo ;

Nishimura, Kenji ;

Shibata, Eriko ;

Tamaki, Masanori ;

Kishi, Fumi ;

Kishi, Seiji ;

Murakami, Taichi ;

Nagai, Kojiro ;

Doi, Toshio .

JOURNAL OF MEDICAL INVESTIGATION, 2018, 65 (3-4) :208-215

[2] Investigating the Value of Urine Volume, Creatinine, and Cystatin C for Urinary Biomarkers Normalization for Drug Development Studies [J].

Adedeji, Adeyemi O. ;

Pourmohamad, Tony ;

Chen, Yafei ;

Burkey, Jennifer ;

Betts, Catherine J. ;

Bickerton, Susan J. ;

Sonee, Manisha ;

McDuffie, James E. .

INTERNATIONAL JOURNAL OF TOXICOLOGY, 2019, 38 (01) :12-22

[3] Comparison of protein, microRNA, and mRNA yields using different methods of urinary exosome isolation for the discovery of kidney disease biomarkers [J].

Alvarez, M. Lucrecia ;

Khosroheidari, Mahdieh ;

Ravi, Rupesh Kanchi ;

DiStefano, Johanna K. .

KIDNEY INTERNATIONAL, 2012, 82 (09) :1024-1032

[4] Method Validation for Preparing Urine Samples for Downstream Proteomic and Metabolomic Applications [J].

Ammerlaan, Wim ;

Trezzi, Jean-Pierre ;

Mathay, Conny ;

Hiller, Karsten ;

Betsou, Fay .

BIOPRESERVATION AND BIOBANKING, 2014, 12 (05) :351-357

[5] Pre-Analytical Handling Conditions and Small RNA Recovery from Urine for miRNA Profiling [J].

Armstrong, David A. ;

Dessaint, John A. ;

Ringelberg, Carol S. ;

Hazlett, Haley F. ;

Howard, Louisa ;

Abdalla, Moemen A. K. ;

Barnaby, Roxanna L. ;

Stanton, Bruce A. ;

Cervinski, Mark A. ;

Ashare, Alix .

JOURNAL OF MOLECULAR DIAGNOSTICS, 2018, 20 (05) :565-571

[6] Advances in lipidomics [J].

Avela, Henri F. ;

Siren, Heli .

CLINICA CHIMICA ACTA, 2020, 510 :123-141

[7] Tumour-derived microvesicles carry several surface determinants and mRNA of tumour cells and transfer some of these determinants to monocytes [J].

Baj-Krzyworzeka, M ;

Szatanek, R ;

Weglarczyk, K ;

Baran, J ;

Urbanowicz, B ;

Branski, P ;

Ratajczak, MZ ;

Zembala, M .

CANCER IMMUNOLOGY IMMUNOTHERAPY, 2006, 55 (07) :808-818

[8] Comparison of urinary extracellular vesicle isolation methods for transcriptomic biomarker research in diabetic kidney disease [J].

Barreiro, Karina ;

Dwivedi, Om Prakash ;

Leparc, German ;

Rolser, Marcel ;

Delic, Denis ;

Forsblom, Carol ;

Groop, Per-Henrik ;

Groop, Leif ;

Huber, Tobias B. ;

Puhka, Maija ;

Holthofer, Harry .

JOURNAL OF EXTRACELLULAR VESICLES, 2020, 10 (02)

[9]

Barreiro K, 2020, METHODS MOL BIOL, V2067, P175, DOI 10.1007/978-1-4939-9841-8_13

[10] Urinary extracellular vesicles. A promising shortcut to novel biomarker discoveries [J].

Barreiro, Karina ;

Holthofer, Harry .

CELL AND TISSUE RESEARCH, 2017, 369 (01) :217-227

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