Using single-cell sequencing technology to detect circulating tumor cells in solid tumors

被引:69
作者
Xu, Jiasheng [1 ,2 ]
Liao, Kaili [3 ]
Yang, Xi [1 ]
Wu, Chengfeng [2 ]
Wu, Wei [4 ]
Han, Shuwen [1 ]
机构
[1] Huzhou Univ, Huzhou Cent Hosp, Dept Oncol, Affiliated Cent Hosp, 1558 Sanhuan North Rd, Huzhou, Zhejiang, Peoples R China
[2] Nanchang Univ, Dept Vasc Surg, Affiliated Hosp 2, 1 Minde Rd, Nanchang 330006, Jiangxi, Peoples R China
[3] Nanchang Univ, Dept Clin Lab, Affiliated Hosp 2, 1 Minde Rd, Nanchang 330006, Jiangxi, Peoples R China
[4] Huzhou Univ, Huzhou Cent Hosp, Dept Gastroenterol, Affiliated Cent Hosp, 1558 Sanhuan North Rd, Huzhou 1558, Zhejiang, Peoples R China
关键词
Circulating tumor cells; Single-cell sequencing; Tumor heterogeneity; Research progress; CANCER-PATIENTS; MUTATION ANALYSIS; GENE-EXPRESSION; DNA-POLYMERASE; COLON-CANCER; RNA-SEQ; HETEROGENEITY; AMPLIFICATION; METASTASES; EVOLUTION;
D O I
10.1186/s12943-021-01392-w
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Circulating tumor cells are tumor cells with high vitality and high metastatic potential that invade and shed into the peripheral blood from primary solid tumors or metastatic foci. Due to the heterogeneity of tumors, it is difficult for high-throughput sequencing analysis of tumor tissues to find the genomic characteristics of low-abundance tumor stem cells. Single-cell sequencing of circulating tumor cells avoids interference from tumor heterogeneity by comparing the differences between single-cell genomes, transcriptomes, and epigenetic groups among circulating tumor cells, primary and metastatic tumors, and metastatic lymph nodes in patients' peripheral blood, providing a new perspective for understanding the biological process of tumors. This article describes the identification, biological characteristics, and single-cell genome-wide variation in circulating tumor cells and summarizes the application of single-cell sequencing technology to tumor typing, metastasis analysis, progression detection, and adjuvant therapy.
引用
收藏
页数:17
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