GENETIC CHARACTERIZATION OF MANGO ACCESSIONS THROUGH RAPD AND ISSR MARKERS IN VIETNAM

Molecular markers have been used to study the genetic diversity in mango (Mangifera indica L.) through characterization worldwide. In this study, 10 RAPD and 10 ISSR markers were used to characterize the genetic richness of 10 mango accessions from different provinces in Vietnam. High polymorphic levels were found in both RAPD (91.9%) and ISSR (98.1%) markers in which a total of 162 and 145 loci were generated, respectively. Both markers revealed high polymorphism information content (PIC) (0.89 for RAPD and 0.91 for ISSR), suggesting that these methods are equally effective for determining genetic variation of mango. However, the Mantel test showed no correlation between these two markers. The dendrograms generated by cluster and PCA analysis of both markers were able to distinguish the cultivars genetically. The obtained results provide molecular biological information for classification, identification plant origins, breeding, and conservation programs; furthermore, utilization of molecular marker analysis could provide new insights to breeders for molecular assisted selection of mango.