Mapping of QTLs for Yield Traits Using F2:3:4 Populations Derived From Two Alien Introgression Lines Reveals qTGW8.1 as a Consistent QTL for Grain Weight From Oryza nivara

被引：6

作者：

Beerelli, Kavitha ^{[1
,2
]}

Balakrishnan, Divya ^{[1
]}

Addanki, Krishnam Raju ^{[1
,2
]}

Surapaneni, Malathi ^{[1
]}

Rao Yadavalli, Venkateswara ^{[1
]}

Neelamraju, Sarla ^{[1
]}

机构：

[1] ICAR Indian Inst Rice Res, Natl Prof Project, Hyderabad, India

[2] Acharya Nagarjuna Univ, Dept Biotechnol, Guntur, Andhra Pradesh, India

来源：

FRONTIERS IN PLANT SCIENCE | 2022年 / 13卷

关键词：

Oryza; alien introgression lines; yield QTL; thousand grain weight; wild species; SATIVA CV SWARNA; NATURAL VARIATION; HEADING DATE; RICE YIELD; LOCI; IDENTIFICATION; ACCESSIONS; DISSECTION; QUALITY; SSR;

D O I：

10.3389/fpls.2022.790221

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Wild introgressions play a crucial role in crop improvement by transferring important novel alleles and broadening allelic diversity of cultivated germplasm. In this study, two stable backcross alien introgression lines 166s and 14s derived from Swarn/Oryza nivara IRGC81848 were used as parents to generate populations to map quantitative trait loci (QTLs) for yield-related traits. Field evaluation of yield-related traits in F-2, F-3, and F-4 population was carried out in normal irrigated conditions during the wet season of 2015 and dry seasons of 2016 and 2018, respectively. Plant height, tiller number, productive tiller number, total dry matter, and harvest index showed a highly significant association to single plant yield in F-2, F-3, and F-4. In all, 21, 30, and 17 QTLs were identified in F-2, F-2:3, and F-2:4, respectively, for yield-related traits. QTLs qPH6.1 with 12.54% phenotypic variance (PV) in F-2, qPH1.1 with 13.01% PV, qTN6.1 with 10.08% PV in F-2:3, and qTGW6.1 with 15.19% PV in F-2:4 were identified as major effect QTLs. QTLs qSPY4.1 and qSPY6.1 were detected for grain yield in F-2 and F-2:3 with PV 8.5 and 6.7%, respectively. The trait enhancing alleles of QTLs qSPY4.1, qSPY6.1, qPH1.1, qTGW6.1, qTGW8.1, qGN4.1, and qTDM5.1 were from O. nivara. QTLs of the yield contributing traits were found clustered in the same chromosomal region. qTGW8.1 was identified in a 2.6 Mb region between RM3480 and RM3452 in all three generations with PV 6.1 to 9.8%. This stable and consistent qTGW8.1 allele from O. nivara can be fine mapped for identification of causal genes. From this population, lines C(2)12, C(2)124, C(2)128, and C(2)143 were identified with significantly higher SPY and C(2)103, C(2)116, and C(2)117 had consistently higher thousand-grain weight values than both the parents and Swarna across the generations and are useful in gene discovery for target traits and further crop improvement.

引用

页数：17

共 80 条

[1]

Ahn S, 2018, PROSTATE INT, V6, P140, DOI [10.1016/j.prnil.2018.01.002, 10.9787/PBB.2018.6.2.140]

[2] Genotype x Environment Interactions of Yield Traits in Backcross Introgression Lines Derived from Oryza sativa cv. Swarna/Oryza nivara [J].

Balakrishnan, Divya ;

Subrahmanyam, Desiraju ;

Badri, Jyothi ;

Raju, Addanki Krishnam ;

Rao, Yadavalli Venkateswara ;

Beerelli, Kavitha ;

Mesapogu, Sukumar ;

Surapaneni, Malathi ;

Ponnuswamy, Revathi ;

Padmavathi, G. ;

Babu, V. Ravindra ;

Neelamraju, Sarla .

FRONTIERS IN PLANT SCIENCE, 2016, 7

[3] Detecting CSSLs and yield QTLs with additive, epistatic and QTL x environment interaction effects from Oryza sativa x O. nivara IRGC81832 cross [J].

Balakrishnan, Divya ;

Surapaneni, Malathi ;

Yadavalli, Venkateswara Rao ;

Addanki, Krishnam Raju ;

Mesapogu, Sukumar ;

Beerelli, Kavitha ;

Neelamraju, Sarla .

SCIENTIFIC REPORTS, 2020, 10 (01)

[4] Genotyping by sequencing of rice interspecific backcross inbred lines identifies QTLs for grain weight and grain length [J].

Bhatia, Dharminder ;

Wing, Rod A. ;

Yu, Yeisoo ;

Chougule, Kapeel ;

Kudrna, Dave ;

Lee, Seunghee ;

Rang, Allah ;

Singh, Kuldeep .

EUPHYTICA, 2018, 214 (02)

[5] Mapping and validation of QTLs for cold tolerance at seedling stage in rice from an indica cultivar Habiganj Boro VI (Hbj. BVI) [J].

Biswas, Partha S. ;

Khatun, Hasina ;

Das, Nomita ;

Sarker, Md. Mahathir ;

Anisuzzaman, M. .

3 BIOTECH, 2017, 7

[6]

Brar DS, 2011, WILD CROP RELATIVES: GENOMIC AND BREEDING RESOURCES: CEREALS, P321, DOI 10.1007/978-3-642-14228-4_7

[7] QTL mapping and introgression of yield-related traits from Oryza glumaepatula to cultivated rice (Oryza sativa) using microsatellite markers [J].

Brondani, C ;

Rangel, PHN ;

Brondani, RPV ;

Ferreira, ME .

THEORETICAL AND APPLIED GENETICS, 2002, 104 (6-7) :1192-1203

[8] Discovery of Consistent QTLs of Wheat Spike-Related Traits under Nitrogen Treatment at Different Development Stages [J].

Deng, Zhiying ;

Cui, Yong ;

Han, Qingdian ;

Fang, Wenqi ;

Li, Jifa ;

Tian, Jichun .

FRONTIERS IN PLANT SCIENCE, 2017, 8

[9]

Doyle JJ., 1990, FOCUS, V12, P13

[10] Quantitative Trait Locus Mapping of the Combining Ability for Yield-Related Traits in Wild Rice Oryza longistaminata [J].

Fan, Fengfeng ;

Long, Weixiong ;

Liu, Manman ;

Yuan, Huanran ;

Pan, Guojing ;

Li, Nengwu ;

Li, Shaoqing .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2019, 67 (32) :8766-8772

← 1 2 3 4 5 6 7 8 →