Differential expression of the chsE gene encoding a chitin synthase of Aspergillus nidulans in response to developmental status and growth conditions

被引：46

作者：

Lee, JI ^{[1
]}

Yu, YM ^{[1
]}

Rho, YM ^{[1
]}

Park, BC ^{[1
]}

Choi, JH ^{[1
]}

Park, HM ^{[1
]}

Maeng, PJ ^{[1
]}

机构：

[1] Chungnam Natl Univ, Sch Biosci & Biotechnol, Dept Microbiol, Taejon 305764, South Korea

来源：

FEMS MICROBIOLOGY LETTERS | 2005年 / 249卷 / 01期

关键词：

Aspergillus nidulans; chsE; chitin synthase; sGFP; differential expression;

D O I：

10.1016/j.femsle.2005.06.006

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Expression of chsE encoding one of the five chitin synthases of Aspergillus nidulans was analyzed. Expression of chsE was moderate in conidiophores, but somewhat weaker in vegetative mycelia. During sexual development, chsE was expressed strongly in young cleistothecia and hulle cells, but little in mature sexual structures. Deletion of chsE caused a significant decrease in the chitin content of the cell wall during early sexual development. Expression of chsE was increased by substituting glucose with lactose or by addition of 0.6 M KCl or NaCl, but affected little by substituting glucose with sodium acetate. Consequently, chsE was shown to have a mode of expression distinct from those of the other chitin synthase genes, chsA, chsB and chsC. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：121 / 129

页数：9

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