Brucella alters endocytic pathway in J774 macrophages

被引:4
作者
Arenas, Graciela N. [1 ,2 ]
Grilli, Diego J. [1 ,2 ]
Samartino, Luis E.
Magadan, Javier [3 ]
Mayorga, Luis S. [3 ]
机构
[1] Univ Nacl Cuyo, Area Microbiol, RA-5500 Mendoza, Argentina
[2] Univ Nacl Cuyo, Inst Histol & Embriol, CONICET, RA-5500 Mendoza, Argentina
[3] Univ Nacl Cuyo, Fac Ciencias Med, RA-5500 Mendoza, Argentina
关键词
brucella; macrophages; endocytic pathway; vaccine strains; virulent strain; intracellular transport; ENDOPLASMIC-RETICULUM; INTRACELLULAR SURVIVAL; LYSOSOME FUSION; ABORTUS; MEMBRANE; REPLICATION; ENDOSOMES; INVASION;
D O I
10.4161/viru.1.5.12861
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Brucella is a facultative intracellular bacterium which causes chronic infections in mammals by surviving and replicating within host cells. The putative role of the endoplasmic reticulum (ER) in the formation of the phagosome in nonprofessional phagocytes is supported by several research groups, but still leaves open the question of the fate of Brucella inside professional phagocytes and its resistance mechanisms therein. Macrophages are particularly important for the survival and spreading of Brucella during infection. The intracellular transport of Brucella in these cells has not been thoroughly characterized. To study the maturation process of Brucella-containing phagosomes in phagocytes, we comparatively monitored the intracellular transport of a virulent strain (2308) with two vaccine strains (S19 and RB51) in J 774 macrophages. Then, we compared the behavior of all three strains studied through transmission electron microscopy. The results indicate that the virulent strain not only occupies two different kinds of compartments but also alters the endocytic pathway of the cell it parasitizes, unlike what has been reported for non-professional phagocytes, like HeLa cell. Besides, differences are observed in the behavior of both Brucella abortus vaccine strains.
引用
收藏
页码:376 / 385
页数:10
相关论文
共 26 条
  • [21] PATHOGENESIS OF ABORTION OF BOVINE BRUCELLOSIS
    SAMARTINO, LE
    ENRIGHT, FM
    [J]. COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES, 1993, 16 (02) : 95 - 101
  • [22] SAMARTINO LE, 1992, AM J VET RES, V53, P359
  • [23] Brucellosis in Argentina
    Samartino, LE
    [J]. VETERINARY MICROBIOLOGY, 2002, 90 (1-4) : 71 - 80
  • [24] INVASION AND REPLICATION OF BRUCELLA-ABORTUS IN 3 DIFFERENT TROPHOBLASTIC CELL-LINES
    SAMARTINO, LE
    TRUAX, RE
    ENRIGHT, FM
    [J]. JOURNAL OF VETERINARY MEDICINE SERIES B-ZENTRALBLATT FUR VETERINARMEDIZIN REIHE B-INFECTIOUS DISEASES AND VETERINARY PUBLIC HEALTH, 1994, 41 (04): : 229 - 236
  • [25] Safe haven: The cell biology of nonfusogenic pathogen vacuoles
    Sinai, AP
    Joiner, KA
    [J]. ANNUAL REVIEW OF MICROBIOLOGY, 1997, 51 : 415 - 462
  • [26] The nature of the phagosomal membrane: endoplasmic reticulum versus plasmalemma
    Touret, N
    Paroutis, P
    Grinstein, S
    [J]. JOURNAL OF LEUKOCYTE BIOLOGY, 2005, 77 (06) : 878 - 885