Highly Segregated Biocomposite Membrane as a Functionally Graded Template for Periodontal Tissue Regeneration

被引:6
作者
Qasim, Syed Saad B. [1 ]
Baig, Mirza Rustum [2 ]
Matinlinna, Jukka Pekka [3 ,4 ]
Daood, Umer [5 ]
Al-Asfour, Adel [6 ]
机构
[1] Kuwait Univ, Fac Dent, Dept Bioclin Sci, POB 24923, Safat 13110, Kuwait
[2] Kuwait Univ, Fac Dent, Dept Restorat Sci, POB 24923, Safat 13110, Kuwait
[3] Univ Hong Kong, Fac Dent, Dent Mat Sci, Appl Oral Sci,Sai Ying Pun, Hosp Rd 34, Hong Kong, Peoples R China
[4] Univ Manchester, Sch Med Sci, Div Dent, Manchester M13 9PL, Lancs, England
[5] Int Med Univ Kuala Lumpur, Fac Dent, Restorat Div, Clin Dent, 126,Jalan Jalil Perkasa 19, Kuala Lumpur 57000, Malaysia
[6] Kuwait Univ, Fac Dent, Dept Surg Sci, POB 24923, Safat 13110, Kuwait
关键词
chitosan; hydroxyapatite; guided tissue regeneration; periodontal engineering; IN-VIVO DEGRADATION; COMPOSITE MEMBRANES; CHITOSAN SCAFFOLDS; CELL-ADHESION; VITRO; BIOMATERIALS; BEHAVIOR; CHITIN; NANOFIBERS; GENIPIN;
D O I
10.3390/membranes11090667
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Guided tissue regeneration (GTR) membranes are used for treating chronic periodontal lesions with the aim of regenerating lost periodontal attachment. Spatially designed functionally graded bioactive membranes with surface core layers have been proposed as the next generation of GTR membranes. Composite formulations of biopolymer and bioceramic have the potential to meet these criteria. Chitosan has emerged as a well-known biopolymer for use in tissue engineering applications due to its properties of degradation, cytotoxicity and antimicrobial nature. Hydroxyapatite is an essential component of the mineral phase of bone. This study developed a GTR membrane with an ideal chitosan to hydroxyapatite ratio with adequate molecular weight. Membranes were fabricated using solvent casting with low and medium molecular weights of chitosan. They were rigorously characterised with scanning electron microscopy, Fourier transform infrared spectroscopy in conjunction with photoacoustic sampling accessory (FTIR-PAS), swelling ratio, degradation profile, mechanical tensile testing and cytotoxicity using human osteosarcoma and mesenchymal progenitor cells. Scanning electron microscopy showed two different features with 70% HA at the bottom surface packed tightly together, with high distinction of CH from HA. FTIR showed distinct chitosan dominance on top and hydroxyapatite on the bottom surface. Membranes with medium molecular weight showed higher swelling and longer degradation profile as compared to low molecular weight. Cytotoxicity results indicated that the low molecular weight membrane with 30% chitosan and 70% hydroxyapatite showed higher viability with time. Results suggest that this highly segregated bilayer membrane shows promising potential to be adapted as a surface layer whilst constructing a functionally graded GTR membrane on its own and for other biomedical applications.
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页数:15
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