Genetic organization and DNA sequence of early region 4 of bovine adenovirus type 3

被引：14

作者：

Lee, JB ^{[1
]}

Baxi, MK ^{[1
]}

Idamakanti, N ^{[1
]}

Reddy, PS ^{[1
]}

Zakhartchouk, AN ^{[1
]}

Pyne, C ^{[1
]}

Babiuk, LA ^{[1
]}

Tikoo, SK ^{[1
]}

机构：

[1] Univ Saskatchewan, VIDO, Virol Grp, Saskatoon, SK S7N 5E3, Canada

来源：

VIRUS GENES | 1998年 / 17卷 / 01期

基金：

加拿大自然科学与工程研究理事会;

关键词：

adenovirus; BAV3; E4; map location;

D O I：

10.1023/A:1008017404513

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have identified and sequenced 3614 nucleotides located at the extreme right-end of the bovine adenovirus type 3 (BAV3) genome from map units 89.5-100. Analysis of the sequence revealed an inverted terminal repeat (ITR) of 195 bp, and identified five open reading frames (ORFs) designated ORF1, ORF2, ORF3, ORF4 and ORF5. When compared with known E4 ORFs of other adenoviruses, ORFs 1, 2 and 4, which code for proteins of 143, 69 and 143 amino acids respectively, were found to be unique to BAV3. ORFs 3 and 5, which code for proteins of 268 and 219 amino acids respectively, showed partial homology to the E4 34 kDa protein of human adenovirus 2. Nucleotide sequence analysis also identified two potential TATA boxes upstream of ORF1 and a potential polyadenylation signal downstream of ORF5 suggesting that E4 transcripts may be 3' co-terminal.

引用

页码：99 / 100

页数：2