Simultaneous electrochemical detection of uric acid and ascorbic acid at a silver doped poly(glutamic acid) modified glassy carbon electrode

被引：14

作者：

Sun, D. M. ^{[1
]}

Hu, W. N. ^{[1
]}

Ma, W. ^{[1
]}

机构：

[1] Huaibei Coal Normal Coll, Dept Chem, Huaibei 235000, Anhui, Peoples R China

来源：

JOURNAL OF ANALYTICAL CHEMISTRY | 2011年 / 66卷 / 03期

关键词：

electrochemical detection; glassy carbon electrode; silver dopped poly(glutamic acid); uric acid; ascorbic acid; SELF-ASSEMBLED MONOLAYERS; CAPILLARY-ELECTROPHORESIS; GOLD ELECTRODE; D-SERINE; OXIDATION; ELECTROANALYSIS; EPINEPHRINE; DOPAMINE; XANTHINE; SENSOR;

D O I：

10.1134/S1061934811030063

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In this paper, the silver doped poly (L-glutamic acid) modified glassy carbon electrode (PLG-Ag/GCE) was fabricated through an electrochemical immobilization. The modified electrode was used for simultaneous determination of uric acid (UA) and ascorbic acid (AA) in 0.1 M phosphate buffer solutions (PBS). The cyclic voltammetric signals of UA and AA were well separated with a potential difference of 396 mV in pH 3.0 phosphate buffer solution. The linear calibration curves were obtained in the concentration range 5.00 x 10(-7)-1.00 x 10(-4) M for UA and 8.00 x 10(-6)-5.00 x 10(-3) M for AA with the detection limits of 3 x 10(-7) M and 4 x 10(-6) M, respectively. The relative standard deviations were 1.3 and 1.0% for the determinations of UA and AA for 20 continuous measurements. The signal was highly stable and reproducible. This method was successfully applied to the determination of UA in human urine samples.

引用

页码：310 / 316

页数：7

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