PLGA-collagen-ECM hybrid meshes mimicking stepwise osteogenesis and their influence on the osteogenic differentiation of hMSCs

被引：25
作者：
Chen, Yazhou [1 ,2 ]
Lee, Kyubae [1 ,2 ]
Yang, Yingnan [3 ]
Kawazoe, Naoki [1 ]
Chen, Guoping [1 ,2 ]
机构：
[1] Natl Inst Mat Sci, Funct Mat Res Ctr, 1-1 Namiki, Tsukuba, Ibaraki 3050044, Japan
[2] Univ Tsukuba, Dept Mat Sci & Engn, Grad Sch Pure & Appl Sci, 1-1-1 Tennodai, Tsukuba, Ibaraki 3058577, Japan
[3] Univ Tsukuba, Grad Sch Life & Environm Sci, 1-1-1 Tennodai, Tsukuba, Ibaraki 3058571, Japan
来源：
BIOFABRICATION | 2020年 / 12卷 / 02期
关键词：
dynamically changed extracellular matrices; human mesenchymal stem cells; stepwise osteogenesis-mimicking; PLGA-collagen-ECM hybrid meshes; MESENCHYMAL STEM-CELLS; EXTRACELLULAR-MATRIX SCAFFOLDS; CHONDROGENIC DIFFERENTIATION; ADIPOGENIC DIFFERENTIATION; PHENOTYPE; ADHESION; PROLIFERATION; EXPRESSION; COCULTURE; MIGRATION;
D O I：
10.1088/1758-5090/ab782b
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
Extracellular matrices (ECMs) are dynamically altered and remodeled during tissue development. How the dynamic remodeling of ECM affects stem cell functions remains poorly understood due to the difficulty of obtaining biomimetic ECMs. In this study, stepwise osteogenesis-mimicking ECM-deposited hybrid meshes were prepared by culturing human mesenchymal stem cells (hMSCs) in poly (lactic-co-glycolic acid) (PLGA)-collagen hybrid meshes and controlling the stages of the osteogenesis of hMSCs. Three types of hybrid mesh mimicking the ECMs that were secreted from stem cell stage of hMSCs (SC-ECM), early stage (EO-ECM) and late stage (LO-ECM) osteogenesis of hMSCs were prepared. The stepwise osteogenesis-mimicking ECM deposited PLGA-collagen hybrid meshes showed different ECM compositions associated with the stage of osteogenesis. Their effects on the osteogenic differentiation of hMSCs differed. EO-ECM scaffold increased and LO-ECM scaffold moderately promoted the osteogenic differentiation of hMSCs. However, SC-ECM scaffold inhibited the osteogenic differentiation of hMSCs. The novel PLGA-collagen-ECM hybrid meshes will provide useful tools for stem cell culture and tissue engineering.
引用
收藏
页数：14
相关论文
共 76 条
[71]   Matrix mechanical plasticity regulates cancer cell migration through confining microenvironments [J].
Wisdom, Katrina M. ;
Adebowale, Kolade ;
Chang, Julie ;
Lee, Joanna Y. ;
Nam, Sungmin ;
Desai, Rajiv ;
Rossen, Ninna Struck ;
Rafat, Marjan ;
West, Robert B. ;
Hodgson, Louis ;
Chaudhuri, Ovijit .
NATURE COMMUNICATIONS, 2018, 9
[72]  
Yamashita H, 2008, DEV GROWTH DIFFER, V50, P97
[73]   Regulation of mesenchymal stem cell functions by micro-nano hybrid patterned surfaces [J].
Yang, Yingjun ;
Wang, Xinlong ;
Huang, Tsung-Chun ;
Hu, Xiaohong ;
Kawazoe, Naoki ;
Tsai, Wei-Bor ;
Yang, Yingnan ;
Chen, Guoping .
JOURNAL OF MATERIALS CHEMISTRY B, 2018, 6 (34) :5424-5434
[74]   Mesenchymal stem cell-derived extracellular matrix enhances chondrogenic phenotype of and cartilage formation by encapsulated chondrocytes in vitro and in vivo [J].
Yang, Yuanheng ;
Lin, Hang ;
Shen, He ;
Wang, Bing ;
Lei, Guanghua ;
Tuan, Rocky S. .
ACTA BIOMATERIALIA, 2018, 69 :71-82
[75]   A Hybrid Peptide Amphiphile Fiber PEG Hydrogel Matrix for 3D Cell Culture [J].
Zhan, Henan ;
Lowik, Dennis W. P. M. .
ADVANCED FUNCTIONAL MATERIALS, 2019, 29 (16)
[76]   Thermally Responsive Microfibers Mediated Stem Cell Fate via Reversibly Dynamic Mechanical Stimulation [J].
Zhang, Jianguang ;
Cheng, Chong ;
Cuellar-Camacho, Jose Luis ;
Li, Mingjun ;
Xia, Yi ;
Li, Wenzhong ;
Haag, Rainer .
ADVANCED FUNCTIONAL MATERIALS, 2018, 28 (47)
12345678