Cell Uptake and Trafficking Behavior of Non-covalent, Coiled-coil Based Polymer-Drug Conjugates

被引：28

作者：

Apostolovic, Bojana ^{[1
,2
]}

Deacon, Samuel P. E. ^{[3
]}

Duncan, Ruth ^{[3
]}

Klok, Harm-Anton ^{[1
,2
]}

机构：

[1] Ecole Polytech Fed Lausanne, Inst Mat, CH-1015 Lausanne, Switzerland

[2] Ecole Polytech Fed Lausanne, Inst Sci & Ingn Chim, Lab Polymeres, CH-1015 Lausanne, Switzerland

[3] Welsh Sch Pharm, Ctr Polymer Therapeut, Cardiff CF10 3XF, S Glam, Wales

来源：

MACROMOLECULAR RAPID COMMUNICATIONS | 2011年 / 32卷 / 01期

基金：

瑞士国家科学基金会;

关键词：

coiled coil; peptides; polymer-drug conjugates; poly(N-(2-hydroxypropyl)methacrylamide) (PHPMA); reversible addition fragmentation chain transfer; synthesis; ENZYMATICALLY DEGRADABLE BONDS; METHOTREXATE; PEPTIDE; SPECIFICITY; PROTEINS; THERAPEUTICS; PHARMACOLOGY; COPOLYMERS; STABILITY; DESIGN;

D O I：

10.1002/marc.201000434

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

This paper reports on the cell uptake and trafficking properties of a series of non-covalent polymer-drug conjugates. These nanomedicines are composed of a poly(N-(2-hydroxypropyl)methacrylamide) backbone functionalized with multiple copies of a drug. The drug moieties are attached to the polymer via a non-covalent, so called coiled coil motif, which is formed by heterodimerization of two complementary peptide strands, one of which is attached to the polymer carrier and the other to the drug. Cytotoxicity and FACS experiments, which were carried out with model anticancer drug or fluorophore conjugates, provided insight into the cell uptake and trafficking behavior of these conjugates.

引用

页码：11 / 18

页数：8