Determination of 1,4-benzodiazepines by high-performance liquid chromatography electrospray tandem mass spectrometry

被引：52
作者：
Kleinschnitz, M [1 ]
Herderich, M [1 ]
Schreier, P [1 ]
机构：
[1] UNIV WURZBURG,LEHRSTUHL LEBENSMITTELCHEM,D-97074 WURZBURG,GERMANY
来源：
JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1996年 / 676卷 / 01期
关键词：
1,4-benzodiazepines; diazepam; N-desmethyldiazepam; nitrazepam; flunitrazepam; medazepam;
D O I：
10.1016/0378-4347(95)00412-2
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A sensitive and selective method to determine diazepam, N-desmethyldiazepam, nitrazepam, flunitrazepam and medazepam in human serum and urine was established employing solid-phase extraction and high-performance liquid chromatography-electrospray tandem mass spectrometry (HPLC-ESI-MS-MS) with the selected reaction monitoring (SRM) mode. Using diazepam-d(5) and N-desmethyldiazepam-d(5) as internal standards, recoveries between 90.4 and 109.7% were determined. The routine quantification limit was set at 2 ng/ml for serum and urine at a signal-to-noise ratio of 10:1.
引用
收藏
页码：61 / 67
页数：7
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