Compartmentalised RNA catalysis in membrane-free coacervate protocells

被引：229

作者：

Drobot, Bjoern ^{[1
]}

Iglesias-Artola, Juan M. ^{[1
]}

Le Vay, Kristian ^{[2
]}

Mayr, Viktoria ^{[2
]}

Kar, Mrityunjoy ^{[1
]}

Kreysing, Moritz ^{[1
]}

Mutschler, Hannes ^{[2
]}

Tang, T-Y Dora ^{[1
]}

机构：

[1] Max Planck Inst Mol Cell Biol & Genet, Pfotenhauerstr 108, D-01307 Dresden, Germany

[2] Max Planck Inst Biochem, Klopferspitz 18, D-82152 Martinsried, Germany

来源：

NATURE COMMUNICATIONS | 2018年 / 9卷

关键词：

CLEAVING HAMMERHEAD RIBOZYMES; IN-VITRO; COMPLEX COACERVATION; PHASE-SEPARATION; VIROID RNA; MODEL; DROPLETS; MOBILITY; PROTEIN; CELLS;

D O I：

10.1038/s41467-018-06072-w

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Phase separation of mixtures of oppositely charged polymers provides a simple and direct route to compartmentalisation via complex coacervation, which may have been important for driving primitive reactions as part of the RNA world hypothesis. However, to date, RNA catalysis has not been reconciled with coacervation. Here we demonstrate that RNA catalysis is viable within coacervate microdroplets and further show that these membrane-free droplets can selectively retain longer length RNAs while permitting transfer of lower molecular weight oligonucleotides.

引用

页数：9

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