Cascade Reaction System Integrating Single-Atom Nanozymes with Abundant Cu Sites for Enhanced Biosensing

被引：189

作者：

Wu, Yu ^{[1
]}

Wu, Jiabin ^{[2
]}

Jiao, Lei ^{[1
]}

Xu, Weiqing ^{[1
]}

Wang, Hengjia ^{[1
]}

Wei, Xiaoqian ^{[1
]}

Gu, Wenling ^{[1
]}

Ren, Guoxi ^{[3
]}

Zhang, Nian ^{[3
]}

Zhang, Qinghua ^{[4
,5
]}

Huang, Liang ^{[2
]}

Gu, Lin ^{[4
,5
]}

Zhu, Chengzhou ^{[1
]}

机构：

[1] Cent China Normal Univ, Coll Chem, Int Joint Res Ctr Intelligent Biosensing Technol, Key Lab Pesticides & Chem Biol,Minist Educ, Wuhan 430079, Hubei, Peoples R China

[2] Huazhong Univ Sci & Technol, Wuhan Natl Lab Optoelect, Wuhan 430074, Hubei, Peoples R China

[3] Chinese Acad Sci, SIMIT, State Key Lab Funct Mat Informat, Shanghai 200050, Peoples R China

[4] Chinese Acad Sci, Beijing Natl Lab Condensed Matter Phys, Beijing 100190, Peoples R China

[5] Chinese Acad Sci, Inst Phys, Beijing 100190, Peoples R China

来源：

ANALYTICAL CHEMISTRY | 2020年 / 92卷 / 04期

基金：

中国国家自然科学基金;

关键词：

PARKINSONS-DISEASE; ACETYLCHOLINE; CATALYSIS; ROUTE;

D O I：

10.1021/acs.analchem.9b05437

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Single-atom nanozymes (SAzymes), as novel nanozymes with atomically dispersed active sites, are of great importance in the development of nanozymes for their high catalytic activities, the maximum utilization efficiency of metal atoms, and the simple model of active sites. Herein, the peroxidase-like SAzymes with high-concentration Cu sites on carbon nanosheets (Cu-N-C) were synthesized through a salt-template strategy. With the densely distributed active Cu atoms (similar to 5.1 wt %), the Cu-N-C SAzymes exhibit remarkable activity to mimic natural peroxidase. Integrating Cu-N-C SAzymes with natural acetylcholinesterase and choline oxidase, three-enzyme-based cascade reaction system was constructed for the colorimetric detection of acetylcholine and organophosphorus pesticides. This work not only provides a strategy to synthesize SAzymes with abundant active sites but also gives some new insights for robust nanozyme biosensing systems.

引用

页码：3373 / 3379

页数：7

共 57 条

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