Reversible conformational change of cytochrome c at a modified gold electrode in methanol

被引：1

作者：

Crilly, Suzanne ^{[1
]}

Magner, Edmond ^{[1
]}

机构：

[1] Univ Limerick, Mat & Surface Sci Inst, Dept Chem & Environm Sci, Limerick, Ireland

来源：

PHYSICAL CHEMISTRY CHEMICAL PHYSICS | 2010年 / 12卷 / 34期

关键词：

CIRCULAR-DICHROISM SPECTRA; PROTEIN SECONDARY STRUCTURE; SELF-ASSEMBLED MONOLAYERS; TEMPERATURE IONIC LIQUIDS; AGAROSE HYDROGEL FILMS; ORGANIC-SOLVENTS; HEME-PROTEINS; DIRECT ELECTROCHEMISTRY; DIELECTRIC-CONSTANT; TRANSFER KINETICS;

D O I：

10.1039/c0cp00350f

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Changes in the secondary structure of cytochrome c on immersion in methanol were monitored using circular dichroism. These changes occurred immediately in methanol, upon re-immersion in aqueous buffer, the secondary structure was restored in ca. 45 min. This method enables the secondary structure of proteins in non-aqueous solvents to be monitored in real time.

引用

页码：10093 / 10097

页数：5

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