Production of the class-specific antibody and development of direct competitive ELISA for multi-residue detection of organophosphorus pesticides

被引：32

作者：

Liu, Bing ^{[1
]}

Ge, Yu ^{[1
]}

Zhang, Yan ^{[1
]}

Song, Yang ^{[1
]}

Lv, Yanyan ^{[1
]}

Wang, Xiaoxue ^{[1
]}

Wang, Shuo ^{[1
]}

机构：

[1] Tianjin Univ Sci & Technol, Key Lab Food Nutr & Safety, Minist Educ China, Tianjin 300457, Peoples R China

来源：

FOOD AND AGRICULTURAL IMMUNOLOGY | 2012年 / 23卷 / 02期

关键词：

ELISA; organophosphorus pesticides; class-specific antibodies; multi-residue; LINKED-IMMUNOSORBENT-ASSAY; MONOCLONAL-ANTIBODIES; GENERIC HAPTEN; IMMUNOASSAYS;

D O I：

10.1080/09540105.2011.608120

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Based on the common structure of organophosphorus pesticides, O,O-dimethyl-phosphorothioate, two haptens were synthesised with different spacer arms. The class-specific polyclonal antisera were prepared from the conjugates of haptens and keyhole limpet hemocyanin. Under optimum conditions, a homologous direct competitive enzyme-linked immunosorbent assay (ELISA) method was developed for multi-residue detection of 23 organophosphate pesticides. The most sensitive IC50 values of the multi-residue analysis were estimated as 0.25, 0.65 and 0.80 mg L-1 for methyl parathion (PM), fenitrothion and fenthion, respectively. Furthermore, when the developed ELISA was applied to detection of PM in water samples, adequate spike recoveries in the range from 70.3 to 131% were obtained. It provided a useful multi-residue determination method of organophophorus pesticides.

引用

页码：157 / 168

页数：12

共 16 条

[1] Use of phosphonic acid as a generic hapten in the production of broad specificity anti-organophosphate pesticide antibody [J].