Development of a monoclonal antibody based competitive-ELISA for detection and titration of antibodies to peste des petits ruminants (PPR) virus

被引:135
作者
Singh, RP [1 ]
Sreenivasa, BP [1 ]
Dhar, P [1 ]
Shah, LC [1 ]
Bandyopadhyay, SK [1 ]
机构
[1] Indian Vet Res Inst, Div Virol, Naini Tal 263138, Uttaranchal, India
关键词
peste des petits ruminants; antibody detection; competitive-ELISA; monoclonal antibody;
D O I
10.1016/j.vetmic.2003.07.007
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Peste des petits ruminants (PPR) is an acute febrile, viral, disease of small ruminants with great economic importance. A competitive-ELISA (c-ELISA) test was developed for detection of antibodies to PPR virus in the sera samples of goats and sheep. The test uses monoclonal antibody to a neutralizing epitope of haemagglutinin protein of the virus. Based on the distribution of known negative sera samples (n = 933) in respect of PPR virus antibodies in the test, a cut-off value was set as 38%. This value was the result of mean of negative population added with two times the standard deviations. A total of 1668 sera samples from goat and sheep and 32 sera from cattle were screened by c-ELISA and virus neutralization test (VNT). Efficacy of c-ELISA compared very well with VNT having high relative specificity (98.4%) and sensitivity (92.4%). The sensitivity of c-ELISA for PPR sero-surveillance could further be increased (95.4%), if the target population is non-vaccinated. c-ELISA test correlated well with VNT (r = 0.845) for end-point titration of PPR virus antibody in 64 goat sera samples. It could clearly separate infected population from uninfected in field sera. Using c-ELISA test paired sera samples from 13 goats provided a clear diagnosis of PPR virus infection. Furthermore, antibodies to PPR virus could be successfully detected during 1 year after vaccination in four goats inoculated with an experimental PPR vaccine. Findings suggest that the c-ELISA test developed can easily replace VNT for sero-surveillance, sero-monitoring, diagnosis from paired sera samples and end-point titration of PPR virus antibodies. (C) 2003 Published by Elsevier B.V.
引用
收藏
页码:3 / 15
页数:13
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