Effects of Lycium barbarum polysaccharides with different molecular weights on function of RAW264.7 macrophages

被引：29
作者：
Deng, Xiangliang [1 ]
Liu, Qi [1 ]
Fu, Yajun [1 ]
Luo, Xia [1 ]
Hu, Minghua [2 ]
Ma, Fangli
Wang, Qing [1 ]
Lai, Xiaoping [3 ]
Zhou, Lian [1 ]
机构：
[1] Guangzhou Univ Chinese Med, Guangzhou Higher Educ Mega Ctr, Sch Pharmaceut Sci, 232 Wai Huan Dong Rd, Guangzhou 510006, Guangdong, Peoples R China
[2] Infinitus Chinese Herbal Immun Res Ctr, Guangzhou, Guangdong, Peoples R China
[3] Guangzhou Univ Chinese Med, Dongguan Math Engn Acad Chinese Med, Dongguan, Peoples R China
来源：
FOOD AND AGRICULTURAL IMMUNOLOGY | 2018年 / 29卷 / 01期
基金：
中国博士后科学基金; 中国国家自然科学基金;
关键词：
Lycium barbarum polysaccharide; molecular weight; macrophages; Griess reagent; flow cytometer; NITRIC-OXIDE; PROTEIN COMPLEX; PHAGOCYTOSIS;
D O I：
10.1080/09540105.2018.1457628
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
The present study was aimed at investigating the effects of four LBP fractions with different molecular weights (MWs), designated LBP2, LBP3, LBP4 and LBP5, on RAW264.7 macrophages function. Results showed that LBP fractions could significantly enhance the expression of CD86 and MHC-II molecules on RAW264.7 macrophages. LBP3, LBP4 and LBP5 could enhance the production of ROS, NO, TNF-a and IL-6, and the phagocytosis of RAW264.7 macrophages. LBP2 with an MW of larger than 350 kDa could only enhance the secretion of TNF-alpha. LBP3 enhanced the RAW264.7 macrophages function in a dose-dependent manner and also enhanced the iNOS mRNA expression in the cells. These results demonstrated that the immunomodulatory activity of LBP on RAW264.7 macrophages was closely related to its MW. It indicated that fractions with an MW smaller than 350 kDa were the main active fractions of LBP in enhancing macrophages function.
引用
收藏
页码：808 / 820
页数：13
相关论文
共 21 条
[1]   Redox Control of Inflammation in Macrophages [J].
Bruene, Bernhard ;
Dehne, Nathalie ;
Grossmann, Nina ;
Jung, Michaela ;
Namgaladze, Dmitry ;
Schmid, Tobias ;
von Knethen, Andreas ;
Weigert, Andreas .
ANTIOXIDANTS & REDOX SIGNALING, 2013, 19 (06) :595-637
[2]   Activation of Macrophages by Polysaccharide-protein Complex from Lycium barbarum L. [J].
Chen, Zhisong ;
Soo, Mei Yun ;
Srinivasan, Nalini ;
Tan, Benny Kwong Huat ;
Chan, Soh Ha .
PHYTOTHERAPY RESEARCH, 2009, 23 (08) :1116-1122
[3]   Antitumor activity of Lycium barbarum polysaccharides with different molecular weights: an in vitro and in vivo study [J].
Deng, Xiangliang ;
Li, Xiangling ;
Luo, Shuang ;
Zheng, Yongyan ;
Luo, Xia ;
Zhou, Lian .
FOOD & NUTRITION RESEARCH, 2017, 61
[4]   Antimicrobial Mechanisms of Macrophages and the Immune Evasion Strategies of Staphylococcus aureus [J].
Flannagan, Ronald S. ;
Heit, Bryan ;
Heinrichs, David E. .
PATHOGENS, 2015, 4 (04) :826-868
[5]   In vitro effect of Pleurotus florida on macrophage cell viability and nitric oxide production [J].
Ghazanfari, Tooba ;
Yaraee, Roya ;
Farahnejad, Zohre ;
Hakimzadeh, Hoda ;
Danialy, Farideh .
FOOD AND AGRICULTURAL IMMUNOLOGY, 2009, 20 (02) :105-110
[6]   CRIg: A macrophage complement receptor required for phagocytosis of circulating pathogens [J].
Helmy, KY ;
Katschke, KJ ;
Gorgani, NN ;
Elliott, JM ;
Diehl, L ;
Scales, SJ ;
Ghilardi, N ;
Campagne, MV .
CELL, 2006, 124 (05) :915-927
[7]   NITRIC-OXIDE - A CYTO-TOXIC ACTIVATED MACROPHAGE EFFECTOR MOLECULE [J].
HIBBS, JB ;
TAINTOR, RR ;
VAVRIN, Z ;
RACHLIN, EM .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1988, 157 (01) :87-94
[8]   Macrophage signaling and respiratory burst [J].
Iles, KE ;
Forman, HJ .
IMMUNOLOGIC RESEARCH, 2002, 26 (1-3) :95-105
[9]   What does the commonly used DCF test for oxidative stress really show? [J].
Karlsson, Markus ;
Kurz, Tino ;
Brunk, Ulf T. ;
Nilsson, Sven E. ;
Frennesson, Christina I. .
BIOCHEMICAL JOURNAL, 2010, 428 :183-190
[10]   Residual NADPH Oxidase and Survival in Chronic Granulomatous Disease. [J].
Kuhns, Douglas B. ;
Alvord, W. Gregory ;
Heller, Theo ;
Feld, Jordan J. ;
Pike, Kristen M. ;
Marciano, Beatriz E. ;
Uzel, Gulbu ;
DeRavin, Suk See ;
Priel, Debra A. Long ;
Soule, Benjamin P. ;
Zarember, Kol A. ;
Malech, Harry L. ;
Holland, Steven M. ;
Gallin, John I. .
NEW ENGLAND JOURNAL OF MEDICINE, 2010, 363 (27) :2600-2610
123