Saikosaponin a Ameliorates LPS-Induced Acute Lung Injury in Mice

被引：114

作者：

Du, Zhi-An ^{[1
]}

Sun, Mei-Na ^{[1
]}

Hu, Zhan-Sheng ^{[1
]}

机构：

[1] Jinzhou Med Univ, Dept Intens Care Units, Affiliated Hosp 1, Jinzhou 121000, Peoples R China

来源：

INFLAMMATION | 2018年 / 41卷 / 01期

关键词：

Saikosaponin a; LPS; lung injury; NLRP3; NLRP3 INFLAMMASOME ACTIVATION; KAPPA-B PATHWAY; EXPRESSION; INVOLVEMENT; MAPK;

D O I：

10.1007/s10753-017-0677-3

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The purpose of this study was to investigate the protective effects of Saikosaponin a (SSa), a triterpene saponin derived from Radix bupleuri, on lipopolysaccharide (LPS)-induced acute lung injury (ALI) using a murine model. The mice were given SSa 1 h after intranasal instillation of LPS. Then, lung histopathological examination, the wet/dry (W/D) ratio, myeloperoxidase (MPO), and inflammatory cytokines in bronchoalveolar lavage fluid (BALF) were detected in this study. The results showed that SSa reduced lung pathological injury induced by LPS. Furthermore, LPS-induced lung W/D ratio, MPO activity, and inflammatory cytokines TNF-alpha and IL-1 beta in BALF were significantly inhibited by SSa. In addition, SSa suppressed LPS-induced NF-kappa B activation and NLRP3 inflammasome expression. In conclusion, we found that SSa played a critical anti-inflammatory effect through inhibition of NF-kappa B and NLRP3 signaling pathways and protected against LPS-induced ALI.

引用

页码：193 / 198

页数：6

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