Amino acid sequence of the Phaseolus vulgaris var ''Fogo na Serra'' inhibitor and interactive surface modeling for the enzyme-inhibitor complex

被引：3

作者：

deCarvalho, PGB

Bloch, C

Morhy, L

daSilva, MCM

deMello, LV

Neshich, G

机构：

[1] UNIV BRASILIA,DEPT BIOL CELULAR,CTR BRASILEIRO SEQUENCIAMENTO PROT,BR-70910 BRASILIA,DF,BRAZIL

[2] UNIV BRASILIA,DEPT QUIM,BR-70970 BRASILIA,DF,BRAZIL

[3] EMBRAPA,CENARGEN,NATL CTR GENET RESOURCES & BIOTECHNOL,BR-70770900 BRASILIA,DF,BRAZIL

来源：

JOURNAL OF PROTEIN CHEMISTRY | 1996年 / 15卷 / 06期

关键词：

chymotrypsin inhibitor; enzyme-inhibitor complex; amino acid sequence; molecular modeling;

D O I：

10.1007/BF01908541

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A trypsin and chymotrypsin inhibitor from seeds of Phaseolus vulgaris var. ''Fogo na Serra'' (PFSI) was purified and its complete amino acid sequence was determined using Edman degradation methods. The inhibitor was found to belong to the Bowman-Birk family of enzymatic inhibitors; it has 82 amino acid residues and a 8.985-kDa molecular mass. The PFSI/alpha-chymotrypsin binary complex has been modeled using the Turkey ovomucoid inhibitor third domain (OMTKY3) bound to alpha-chymotrypsin [Fujinaga et al. (1987), J. Mol. Biol., 195, 397-418. template. The model allowed identification of the binding surface.

引用

页码：591 / 598

页数：8

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