Mannosylated chitosan-zinc sulphide nanocrystals as fluorescent bioprobes for targeted cancer imaging

被引：43

作者：

Jayasree, Aswathy

Sasidharan, Sajith

Koyakutty, Manzoor

Nair, Shantikumar

Menon, Deepthy ^{[1
]}

机构：

[1] Amrita Vishwavidyapeetham Univ, Amrita Ctr Nanosci & Mol Med, Amrita Inst Med Sci, Cochin 682041, Kerala, India

来源：

CARBOHYDRATE POLYMERS | 2011年 / 85卷 / 01期

关键词：

Bioimaging; Chitosan; D-Mannose; Mannose receptor; Nanocrystals; Oral cancer; Zinc sulphide; QUANTUM DOTS; ACID; NANOPARTICLES; CYSTEINE; MANNOSE; CHITIN;

D O I：

10.1016/j.carbpol.2011.01.034

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A novel nanomaterial based on chitosan-zinc sulphide:Mn (ZnS:Mn) conjugated with mannose ligand has been developed for targeted cancer imaging. The nanobioconjugates, prepared through simple aqueous chemistry, possessed high colloidal stability and strong fluorescence emission at similar to 600 nm. Characterization using XRD, DLS, SEM, AFM and FTIR revealed that the bioconjugated particles are appropriately functionalized and stable, with average size similar to 150 nm. The presence of polysaccharide chitosan bestowed enhanced biocompatibility to the nanocrystals and provided suitable functionality for mannosylation. In vitro cytotoxicity studies on mouse fibroblast (L929) and oral epithelial carcinoma (KB) cells confirmed their cytocompatibility. Bioconjugation with mannose provided specificity and targeted cellular labelling characteristics as demonstrated using KB cells which over-express mannose receptors on their surface. Our investigations highlight the applicability of polysaccharide protected and mannosylated fluorescent ZnS nanoprobes for active targeting of cancer cells. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：37 / 43

页数：7

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