The effects of Brazilian green propolis that contains flavonols against mutant copper-zinc superoxide dismutase-mediated toxicity

被引：51

作者：

Ueda, Tomoyuki ^{[1
]}

Inden, Masatoshi ^{[1
]}

Shirai, Katsuhiro ^{[1
]}

Sekine, Shin-ichiro ^{[1
]}

Masaki, Yuji ^{[1
]}

Kurita, Hisaka ^{[1
]}

Ichihara, Kenji ^{[2
]}

Inuzuka, Takashi ^{[3
]}

Hozumi, Isao ^{[1
]}

机构：

[1] Gifu Pharmaceut Univ, Lab Med Therapeut & Mol Therapeut, Gifu, Japan

[2] Api Co Ltd, Nagaragawa Res Ctr, Gifu, Japan

[3] Gifu Univ, Dept Neurol & Geriatr, Grad Sch Med, Gifu, Japan

来源：

SCIENTIFIC REPORTS | 2017年 / 7卷

关键词：

AMYOTROPHIC-LATERAL-SCLEROSIS; SPINAL-CORD; SOD1; AUTOPHAGY; ACCUMULATION; ANTIOXIDANT; PROTEASOME; PATHWAY; STRESS; CELLS;

D O I：

10.1038/s41598-017-03115-y

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease characterized by the selective and progressive loss of motor neurons. The purpose of this study was to clarify effects of brazilian green propolis and the active ingredient against ALS-associated mutant copper-zinc superoxide dismutase (SOD1)-mediated toxicity. Ethanol extract of brazilian green propolis (EBGP) protected N2a cells against mutant SOD1-induced neurotoxicity and reduced aggregated mutant SOD1 by induction of autophagy. Kaempferide and kaempferol, the active ingredients of EBGP, also inhibited mutant SOD1-induced cell death and reduced the intracellular mutant SOD1 aggregates. Both kaempferide and kaempferol significantly suppressed mutant SOD1-induced superoxide in mitochondria. Western blot analysis showed that kaempferol potentially induced autophagy via the AMP-activated protein kinase (AMPK) - the mammalian target of rapamycin (mTOR) pathway. These results suggest that EBGP containing the active ingredient against mutant SOD1-mediated toxicity is a promising medicine or health food for prevention and treatment of ALS.

引用

页数：11