The expression pattern of ADP-ribosyltransferase 3 in rat traumatic brain injury

被引:15
作者
Shi, Wei [1 ]
Gong, Peipei [1 ]
Fan, Jian [2 ]
Yan, Yao Hua [1 ]
Ni, Lanchun [1 ]
Wu, Xiaohong [1 ]
Cui, Gang [1 ]
Wu, Xinmin [1 ]
Gu, Xingxing [1 ]
Chen, Jian [1 ]
机构
[1] Nantong Univ, Dept Neurosurg, Surg Comprehens Lab, Affiliated Hosp, Nantong 226001, Jiangsu, Peoples R China
[2] Nantong Univ, Dept Neurosurg, Affiliated Qidong People Hosp, Nantong 226200, Jiangsu, Peoples R China
基金
中国国家自然科学基金;
关键词
ART3; Traumatic brain injury; Apoptosis; GAP43; Neurons; Rat; DIFFUSE AXONAL INJURY; GLIAL PROLIFERATION; RIBOSYLATION; NEUROGENESIS; SUBSTRATE; APOPTOSIS; TOXIN; NEUROPROTECTION; MECHANISMS; INHIBITION;
D O I
10.1007/s10735-011-9366-y
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Mammalian ecto ADP-ribosyltransferases (ARTs) can regulate the biological functions of various types of cells by catalyzing the transfer of single ADP-ribose moiety from NAD(+) to a specific amino acid in a target protein. ART3 is a member of the known ART family which is involved in cell division, DNA-repair and the regulation of the inflammatory response. To elucidate the expression, cellular localization and possible functions of ART3 in central nervous system (CNS) lesion and repair, we performed an acute traumatic brain injury model in adult rats. Western blot analysis showed that the expression of ART3 in ipsilateral brain cortex increased, then reached a peak at day 3 after traumatic brain injury (TBI), and gradually declined during the following days. But in the contralateral brain cortex, no obvious alterations were observed. Immunohistochemistry revealed the highly significant accumulation of ART3 at the ipsilateral brain in comparison to contralateral cerebral cortex. Double immunofluorescence labeling suggested that ART3 was localized mainly in the plasmalemma of neurons, but not in astrocytes or microglias within 3 mm from the lesion site at day 3 post-injury. In addition, we detected the expression profiles of caspase-3 and growth associated protein 43 (GAP-43) whose changes were correlated with the expression profiles of ART3 in this TBI model. Besides, co-localization of ART3/active caspase-3 and ART3/GAP43 were detected in NeuN-positive cells, respectively. Moreover, Pheochromocytoma (PC12) cells were treated with H2O2 to establish an apoptosis model. The results showed that the expression of ART3 was increased in the concentration and time dependence way. To further examine the involvement of ART3 in apoptosis of PC12, 3-Methoxybenzamide was used in flow cytometry analysis of apoptotic cells stained with Annexin V and PI. The experimental group in which 3-Methoxybenzamide used had a relative low level of apoptotic index compared with the untreated group. Together with previous reports, we hypothesize that ART3 may play important roles in CNS pathophysiology after TBI and further research is needed to have a good understanding of its function and mechanism.
引用
收藏
页码:37 / 47
页数:11
相关论文
共 45 条
[1]   Rapid induction of naive T cell apoptosis by ecto-nicotinamide adenine dinucleotide: Requirement for mono(ADP-ribosyl)Transferase 2 and a downstream effector [J].
Adriouch, S ;
Ohlrogge, W ;
Haag, F ;
Koch-Nolte, F ;
Seman, M .
JOURNAL OF IMMUNOLOGY, 2001, 167 (01) :196-203
[2]   Expression and selective up-regulation of toxin-related mono ADP-ribosyltransferases by pathogen-associated molecular patterns in alveolar epithelial cells [J].
Balducci, Enrico ;
Micossi, Luigi G. ;
Soldaini, Elisabetta ;
Rappuoli, Rino .
FEBS LETTERS, 2007, 581 (22) :4199-4204
[3]  
BENOWITZ LI, 1991, PROG BRAIN RES, V89, P69
[4]   Molecular characterization and expression of the gene for mouse NAD+:arginine ecto-mono(ADP-ribosyl)transferase, Art1 [J].
Braren, R ;
Glowacki, G ;
Nissen, M ;
Haag, F ;
Koch-Nolte, F .
BIOCHEMICAL JOURNAL, 1998, 336 :561-568
[5]   Neural apoptosis [J].
Bredesen, DE .
ANNALS OF NEUROLOGY, 1995, 38 (06) :839-851
[6]   Characterization of a prolonged regenerative attempt by diffusely injured axons following traumatic brain injury in adult cat: a light and electron microscopic immunocytochemical study [J].
Christman, CW ;
Salvant, JB ;
Walker, SA ;
Povlishock, JT .
ACTA NEUROPATHOLOGICA, 1997, 94 (04) :329-337
[7]   Caspase-3 mediated neuronal death after traumatic brain injury in rats [J].
Clark, RSB ;
Kochanek, PM ;
Watkins, SC ;
Chen, MZ ;
Dixon, CE ;
Seidberg, NA ;
Melick, J ;
Loeffert, JE ;
Nathaniel, PD ;
Jin, KL ;
Graham, SH .
JOURNAL OF NEUROCHEMISTRY, 2000, 74 (02) :740-753
[8]   NEUROGRANIN, A B-50/GAP-43-IMMUNOREACTIVE C-KINASE SUBSTRATE (BICKS), IS ADP-RIBOSYLATED [J].
COGGINS, PJ ;
MCLEAN, K ;
ZWIERS, H .
FEBS LETTERS, 1993, 335 (01) :109-113
[9]   Functional aspects of protein mono-ADP-ribosylation [J].
Corda, D ;
Di Girolamo, M .
EMBO JOURNAL, 2003, 22 (09) :1953-1958
[10]   NEUROMODULIN (GAP43) - A NEURONAL PROTEIN-KINASE-C SUBSTRATE IS ALSO PRESENT IN 0-2A GLIAL-CELL LINEAGE - CHARACTERIZATION OF NEUROMODULIN IN SECONDARY CULTURES OF OLIGODENDROCYTES AND COMPARISON WITH THE NEURONAL ANTIGEN [J].
DELOULME, JC ;
JANET, T ;
AU, D ;
STORM, DR ;
SENSENBRENNER, M ;
BAUDIER, J .
JOURNAL OF CELL BIOLOGY, 1990, 111 (04) :1559-1569