Placental Sequestration of Plasmodium falciparum Malaria Parasites Is Mediated by the Interaction Between VAR2CSA and Chondroitin Sulfate A on Syndecan-1

被引：69

作者：

Pereira, Marina Ayres ^{[1
,2
]}

Clausen, Thomas Mandel ^{[1
,2
,3
]}

Pehrson, Caroline ^{[1
,2
]}

Mao, Yang ^{[4
,5
,6
]}

Resende, Mafalda ^{[1
,2
]}

Daugaard, Mads ^{[3
]}

Kristensen, Anders Riis ^{[7
]}

Spliid, Charlotte ^{[1
,2
]}

Mathiesen, Line ^{[8
]}

Knudsen, Lisbeth E. ^{[8
]}

Damm, Peter ^{[9
]}

Theander, Thor G. ^{[1
,2
]}

Hansson, Stefan R. ^{[10
]}

Nielsen, Morten A. ^{[1
,2
]}

Salanti, Ali ^{[1
,2
]}

机构：

[1] Univ Copenhagen, Dept Immunol & Microbiol, Ctr Med Parasitol, DK-1168 Copenhagen, Denmark

[2] Copenhagen Univ Hosp, Copenhagen, Denmark

[3] Vancouver Prostate Ctr, Vancouver, BC, Canada

[4] Boston Univ, Sch Med, Dept Biochem, Boston, MA 02118 USA

[5] Univ Copenhagen, Copenhagen Ctr Glyc, DK-1168 Copenhagen, Denmark

[6] Univ Copenhagen, Dept Cellular & Mol Med, DK-1168 Copenhagen, Denmark

[7] British Columbia Canc Agcy, Michael Smith Genome Sci Ctr, Vancouver, BC, Canada

[8] Univ Copenhagen, Environm Hlth Sect, Dept Publ Hlth, Copenhagen, Denmark

[9] Univ Copenhagen, Rigshosp, Dept Obstet, Fac Hlth & Med Sci, Copenhagen, Denmark

[10] Lund Univ, Univ Lund Hosp, Div Obstet & Gynecol, Dept Clin Sci, Lund, Sweden

来源：

PLOS PATHOGENS | 2016年 / 12卷 / 08期

关键词：

SRC FAMILY KINASES; INFECTED ERYTHROCYTES; ALPHA(5)BETA(1) INTEGRIN; GLYCOSAMINOGLYCAN CHAINS; MULTIPLE ROLES; CELL-LINE; EXPRESSION; BINDING; PROTEIN; PREGNANCY;

D O I：

10.1371/journal.ppat.1005831

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

During placental malaria, Plasmodium falciparum infected erythrocytes sequester in the placenta, causing health problems for both the mother and fetus. The specific adherence is mediated by the VAR2CSA protein, which binds to placental chondroitin sulfate (CS) on chondroitin sulfate proteoglycans (CSPGs) in the placental syncytium. However, the identity of the CSPG core protein and the cellular impact of the interaction have remain elusive. In this study we identified the specific CSPG core protein to which the CS is attached, and characterized its exact placental location. VAR2CSA pull-down experiments using placental extracts from whole placenta or syncytiotrophoblast microvillous cell membranes showed three distinct CSPGs available for VAR2CSA adherence. Further examination of these three CSPGs by immunofluorescence and proximity ligation assays showed that syndecan-1 is the main receptor for VAR2CSA mediated placental adherence. We further show that the commonly used placental choriocarcinoma cell line, BeWo, express a different set of proteoglycans than those present on placental syncytiotrophoblast and may not be the most biologically relevant model to study placental malaria. Syncytial fusion of the BeWo cells, triggered by forskolin treatment, caused an increased expression of placental CS-modified syndecan-1. In line with this, we show that rVAR2 binding to placental CS impairs syndecan-1-related Src signaling in forskolin treated BeWo cells, but not in untreated cells.

引用

页数：26

共 68 条

[1] Characterization of proteoglycans of human placenta and identification of unique chondroitin sulfate proteoglycans of the intervillous spaces that mediate the adherence of Plasmodium falciparum-infected erythrocytes to the placenta [J].