Vasoactive intestinal polypeptide, an erectile neurotransmitter, improves erectile function more significantly in castrated rats than in normal rats

被引：12
作者：
Zhang, Min-Guang [1 ]
Shen, Zhou-Jun [1 ]
Zhang, Cun-Ming [1 ]
Wu, Wei [2 ]
Gao, Ping-Jin [3 ,4 ]
Chen, Shan-Wen [5 ]
Zhou, Wen-Long [1 ]
机构：
[1] Shanghai Jiao Tong Univ, Sch Med, Ruijin Hosp, Dept Urol, Shanghai 200025, Peoples R China
[2] Shanghai Jiao Tong Univ, Sch Med, Ruijin Hosp, Dept Gen Surg, Shanghai 200025, Peoples R China
[3] Chinese Acad Sci, Shanghai Inst Biol Sci, Beijing 100864, Peoples R China
[4] Shanghai Jiao Tong Univ, Sch Med, Inst Hlth Sci, Shanghai 200025, Peoples R China
[5] Zhejiang Univ, Dept Urol, Sch Med, Affiliated Hosp 1, Hangzhou, Zhejiang, Peoples R China
来源：
BJU INTERNATIONAL | 2011年 / 108卷 / 03期
基金：
中国国家自然科学基金;
关键词：
androgen; vasoactive intestinal polypeptide; erectile function; castration; signaling pathway; CORPUS CAVERNOSUM; PENILE ERECTION; SMOOTH-MUSCLE; GENE-EXPRESSION; HYPOGONADAL MEN; NITRIC-OXIDE; DYSFUNCTION; ANDROGEN; VIP; TESTOSTERONE;
D O I：
10.1111/j.1464-410X.2010.09901.x
中图分类号：
R5 [内科学]; R69 [泌尿科学（泌尿生殖系疾病）];
学科分类号：
1002 ; 100201 ;
摘要：
OBJECTIVE To investigate the regulatory role of androgen in VIP-mediated erectile effect. Androgen is essential for physiological erection. Vasoactive intestinal polypeptide (VIP) is an important erectile neurotransmitter. While previous studies demonstrated that VIP expression in the penis was androgen-independent, it remains controversial whether androgen has any effect on VIP-mediated erection. MATERIALS AND METHODS Male SD rats were divided into a control group, a castration group, and a castration-with-testosterone-replacement group. Four weeks later, each group was subdivided into low and high-dose VIP subgroups and subjected to intracavernous injection of 0.5 and 2 mu g VIP, respectively. Erectile function was tested by recording intracavernosal pressure (ICP) and mean arterial blood pressure (MAP) before and after VIP injection. The expressions of the VIP-receptor (VPAC2), G-protein stimulatory and inhibitory alpha subunits (Gs-alpha, Gi-alpha), and PDE3A in rat corpus cavernosum (CC) was qualified by real-time PCR and Western blot analysis. RESULTS Castration reduced erectile function while testosterone restored it. VIP improved erectile function in a dose-dependent manner. High-dose VIP significantly enhanced erectile function in castrated rats and there was no difference of ICP/MAP among three groups after injection of high-dose VIP. Low-dose VIP also resulted in a higher improvement of erectile function in castrated rats, although the ICP/MAP was lower in these rats than in the other two groups. VPAC2 and Gs-alpha were up-regulated while Gi-alpha and PDE3A were down-regulated in CC of castrated rats. CONCLUSION VIP improves erectile function much more significantly in hypogonadal condition, mainly due to the higher expression of VPAC2, Gs-alpha, and lower expression of Gi-alpha and PDE3A in CC of castrated rats. Androgen may negatively regulate the erectile effect of VIP.
引用
收藏
页码：440 / 446
页数：7
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