Crystal structure of human germline antibody 3-23/B3

被引：10

作者：

Malia, Thomas J. ^{[1
]}

Obmolova, Galina ^{[1
]}

Almagro, Juan Carlos ^{[1
]}

Gilliland, Gary L. ^{[1
]}

Teplyakov, Alexey ^{[1
]}

机构：

[1] Centocor R&D Inc, Radnor, PA 19087 USA

来源：

MOLECULAR IMMUNOLOGY | 2011年 / 48卷 / 12-13期

关键词：

Antibody; Germline; Crystal structure; REGIONS; DOMAIN; CLASSIFICATION; HOMOLOGY; PACKING; CDR-H3; MODEL; VH;

D O I：

10.1016/j.molimm.2011.04.020

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The human antibody repertoire is dominated by a few combinations of germline sequences, with the genes 3-23 for the heavy chain and B3 for the light chain being among the most frequently used. Despite this fact, there was no experimental structure of the antibody composed of 3-23 and B3. The crystal structure of the Fab fragment of the synthetic antibody composed of the 3-23 and B3 germline sequences was determined to provide a template for antibody modeling. The antigen-binding loops were found in the canonical conformations. Comparison to the other structures where either 3-23 or B3 is paired with a different chain reveals a significant deviation in the orientation of the variable domains. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：1586 / 1588

页数：3

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