Hydrophobic interaction chromatography of proteins

被引：316
|
作者：
Queiroz, JA [1 ]
Tomaz, CT
Cabral, JMS
机构：
[1] Univ Beira Interior, Dept Quim, P-6201001 Covilha, Portugal
[2] Inst Super Tecn, Ctr Engn Biol & Quim, P-1049001 Lisbon, Portugal
来源：
JOURNAL OF BIOTECHNOLOGY | 2001年 / 87卷 / 02期
关键词：
hydrophobic interaction chromatography; proteins; purification;
D O I：
10.1016/S0168-1656(01)00237-1
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
In this article. an overview of hydrophobic interaction chromatography (HIC) of proteins is given. After a brief description of protein hydrophobicity and hydrophobic interactions, we present the different proposed theories for the retention mechanism of proteins in HIC. Additionally, the main parameters to consider for the optimization of fractionation processes by HIC and the stationary phases available were described. Selected examples of protein fractionation by HIC are also presented. (C) 2001 Elsevier Science B.V. All rights reserved.
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页码：143 / 159
页数：17
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