Puia dinghuensis gen. nov., sp nov., isolated from monsoon evergreen broad-leaved forest soil

A Gram-stain-negative, rod-shaped, aerobic, non-motile bacterial strain, 4GSH07(T), was originally isolated from the monsoon evergreen broad-leaved forest soil of Dinghushan Biosphere Reserve, Guangdong Province, PR China (112 degrees 31 ' E 23 degrees 10 ' N). It grew optimally at 28-33 degrees C and pH 4.0. The 16S rRNA gene sequence analysis showed that strain 4GSH07(T) had the highest sequence similarity of 94.0% to Parasegetibacter terrae JCM 19942(T), and formed an independent lineage separable from other described genera of the family Chitinophagaceae. The main fatty acids (>5 %) were iso-C-15:0, iso-C-17:0 3-OH, C-16:0 3-OH, summed feature 3 (C-16:1 omega 6c and/or C-16:1 omega 7c) and C-15:0 2-OH. The organism contained MK-7 as the predominant respiratory quinone, and the total DNA G+C content was 50.3 mol%. The phenotypic, chemotaxonomic and phylogenetic data showed consistently that strain 4GSH07(T) represents a novel species of a novel genus of the family Chitinophagaceae, for which the name Puia dinghuensis gen. nov., sp. nov., is proposed, with 4GSH07(T) (=CGMCC 1.15448(T) =LMG 29214(T)) as the type strain.