Effect of Porcine Akirin2 on Skeletal Myosin Heavy Chain Isoform Expression

被引：10

作者：

Chen, Xiaoling ^{[1
]}

Luo, Yanliu ^{[1
]}

Zhou, Bo ^{[1
]}

Huang, Zhiqing ^{[1
]}

Jia, Gang ^{[1
]}

Liu, Guangmang ^{[1
]}

Zhao, Hua ^{[1
]}

Yang, Zhouping ^{[2
]}

Zhang, Ruinan ^{[1
]}

机构：

[1] Sichuan Agr Univ, Inst Anim Nutr, Key Lab Anim Dis Resistance Nutr China, Minist Educ, Chengdu 611130, Sichuan, Peoples R China

[2] Sichuan Agr Univ, Coll Sci, Chengdu 611130, Sichuan, Peoples R China

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2015年 / 16卷 / 02期

基金：

中国国家自然科学基金;

关键词：

MUSCLE-FIBER CHARACTERISTICS; MEAT QUALITY TRAITS; POSTMORTEM METABOLIC-RATE; GENE-EXPRESSION; HISTOCHEMICAL-CHARACTERISTICS; CALCINEURIN; ACTIVATION; PATHWAYS; PROTEINS; FAMILY;

D O I：

10.3390/ijms16023996

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Akirin2 plays an important role in skeletal myogenesis. In this study, we found that porcine Akirin2 (pAkirin2) mRNA level was significantly higher in fast extensor digitorum longus (EDL) and longissimus lumborum (LL) muscles than in slow soleus (SOL) muscle of pigs. Overexpression of pAkirin2 increased the number of myosin heavy chain (MHC)-positive cells, indicating that pAkirin2 promoted myoblast differentiation. We also found that overexpression of pAkirin2 increased the mRNA expressions of MHCI and MHCIIa and decreased the mRNA expression of MHCIIb. Myocyte enhancer factor 2 (MEF2) and nuclear factor of activated T cells (NFAT) are the major downstream effectors of calcineurin. Here we also observed that the mRNA expressions of MEF2C and NFATc1 were notably elevated by pAkirin2 overexpression. Together, our data indicate that the role of pAkirin2 in modulating MHCI and MHCIIa expressions may be achieved through calcineurin/NFATc1 signaling pathway.

引用

页码：3996 / 4006

页数：11

共 39 条

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