Interaction of amphotericin B lipid formulations and triazoles with human polymorphonuclear leucocytes for antifungal activity against Zygomycetes

被引：27
作者：
Simitsopoulou, Maria [2 ]
Roilides, Emmanuel [1 ,2 ]
Maloukou, Avgi [2 ]
Gil-Lamaignere, Cristina [2 ]
Walsh, Thomas J. [1 ]
机构：
[1] NCI, Pediat Oncol Branch, Immunocompromised Host Sect, Bethesda, MD 20892 USA
[2] Aristotle Univ Thessaloniki, Hippokration Hosp, Dept Pediat 3, Infect Dis Lab, Thessaloniki, Greece
来源：
MYCOSES | 2008年 / 51卷 / 02期
基金：
美国国家卫生研究院;
关键词：
Zygomycetes; lipid formulations of amphotericin B; triazoles; polymorphonuclear leucocytes;
D O I：
10.1111/j.1439-0507.2007.01457.x
中图分类号：
R75 [皮肤病学与性病学];
学科分类号：
100206 ;
摘要：
The frequency of zygomycosis has increased considerably over recent years mainly in immunocompromised and diabetic patients. Little is known about the effects of host innate immunity against different Zygomycetes especially under the influence of antifungal agents. The antifungal activity of human polymorphonuclear leucocytes (PMN) in combination with liposomal amphotericin B (LAMB), amphotericin B lipid complex (ABLC), voriconazole (VRC) and posaconazole (PSC) against Rhizopus oryzae and Rhizopus microsporus, frequently isolated Zygomycetes, were studied and compared with Absidia corymbifera, a less pathogenic Zygomycete. Antifungal activity was evaluated as per cent of hyphal damage using the XTT metabolic assay. While A. corymbifera was more susceptible to PMN than the other two Zygomycetes, R. microsporus appeared to be the most susceptible to combined effects of amphotericin B formulations and VRC with PMN. LAMB exhibited synergistic activity with PMN in inducing hyphal damage to R. microsporus but not to the other fungi. In contrast, ABLC exhibited synergistic or additive activity with PMN against all three fungi. Among triazoles, only VRC exhibited additive effect with PMN against R. microsporus. Lipid formulations of amphotericin B and particularly ABLC interact with PMN predominantly in inducing augmented hyphal damage to three different species of Zygomycetes.
引用
收藏
页码：147 / 154
页数：8
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