Application of phage-displayed single chain antibodies in western blot

被引：0
|
作者：
Ding, YL
Liu, MY
Han, W
Yang, SL
Liu, H [1 ]
Gong, Y
机构：
[1] Chinese Acad Sci, Shanghai Res Ctr Biotechnol, Shanghai 200233, Peoples R China
[2] Shenyang Pharmaceut Univ, Sch Pharmaceut Engn, Shenyang 110015, Peoples R China
来源：
ACTA BIOCHIMICA ET BIOPHYSICA SINICA | 2005年 / 37卷 / 03期
关键词：
phage-display; anitbody; Western blot;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A phage display single chain fragment variable library constructed on pIII protein of M13 filamentous phage was screened using B-lymphocyte stimulator and FP248 as selective molecules. After four rounds of panning, there was a remarkable enrichment in the titer of bound phages. Twenty phage clones were selected from the last round and screened by means of phage-ELISA. With the antibody phages as primary antibodies in Western blot, we developed a method for detecting the specific antigen. The dilutions of antibody phages depend on the affinity between antibody-displayed phage particles and antigens.
引用
收藏
页码：205 / 209
页数：5
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