A simple and sensitive fluorescence based biosensor for the determination of uric acid using H2O2-sensitive quantum dots/dual enzymes

被引：158
|
作者：
Azmi, Nur Ellina [1 ,2 ]
Ramli, Noor Izaanin [1 ]
Abdullah, Jaafar [2 ,3 ]
Hamid, Mohammad Azmi Abdul [4 ]
Sidek, Hamidah [1 ]
Abd Rahman, Samsulida [1 ,3 ]
Ariffin, Nurhayati [1 ]
Yusof, Nor Azah [2 ,3 ]
机构：
[1] SIRIM Berhad, Ind Biotechnol Res Ctr, Shah Alam 40700, Selangor DE, Malaysia
[2] Univ Putra Malaysia, Inst Adv Technol, Serdang 43400, Selangor DE, Malaysia
[3] Univ Putra Malaysia, Fac Sci, Dept Chem, Upm Serdang 43400, Selangor DE, Malaysia
[4] Univ Kebangsaan Malaysia, Fac Sci & Technol, Sch Appl Phys, Bangi 43600, Selangor DE, Malaysia
来源：
BIOSENSORS & BIOELECTRONICS | 2015年 / 67卷
关键词：
Quantum dots; Enzyme; Uric acid; Fluorescence; Quenching; ASCORBIC-ACID; DOTS; ELECTRODE; DOPAMINE; SERUM;
D O I：
10.1016/j.bios.2014.07.056
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
A novel optical detection system consisting of combination of uricase/HRP-CdS quantum dots (QDs) for the determination of uric acid in urine sample is described. The QDs was used as an indicator to reveal fluorescence property of the system resulting from enzymatic reaction of uricase and HRP (horseradish peroxidase), which is involved in oxidizing uric acid to allaintoin and hydrogen peroxide. The hydrogen peroxide produced was able to quench the QDs fluorescence, which was proportional to uric acid concentration. The system demonstrated sufficient activity of uricase and HRP at a ratio of 5U:5U and pH 7.0. The linearity of the system toward uric acid was in the concentration range of 125-1000 mu M with detection limit of 125 mu M. (C) 2014 Elsevier B.V. All rights reserved.
引用
收藏
页码：129 / 133
页数：5
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