Photodynamic Therapy Potentiates the Effects of Curcumin on Pediatric Epithelial Liver Tumor Cells

被引：1
作者：
Ellerkamp, Verena [1 ]
Bortel, Nicola [1 ]
Schmid, Evi [1 ]
Kirchner, Bettina [1 ]
Armeanu-Ebinger, Sorin [1 ]
Fuchs, Joerg [1 ]
机构：
[1] Univ Hosp Tuebingen, Dept Pediat Surg & Pediat Urol, Tubingen, Germany
来源：
ANTICANCER RESEARCH | 2016年 / 36卷 / 07期
关键词：
Curcumin; photodynamic therapy; heptoblastoma; hepatocellular carcinoma; INTERNATIONAL-SOCIETY; VISIBLE-LIGHT; HEPATOBLASTOMA; LINE; PHOTOCYTOTOXICITY; COMBINATION; ACTIVATION; CARCINOMA; CHILDREN; GROWTH;
D O I：
暂无
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Background/Aim: Curcumin (CUM) is a promising agent in complementary oncology. The present study analyzed the photoactive properties of curcumin on pediatric epithelial liver tumor cell lines. Materials and Methods: Hepatoblastoma cell lines (HuH6, HepT1) and hepatocellular carcinoma cell lines (HepG2, HC-AFW1) were treated with curcumin and exposed to blue light (phototherapy, 480 nm, 300 W). Cell viability (MTT tests), cellular oxidative stress (production of reactive oxygen species (ROS)) and cellular uptake/degradation of curcumin were analyzed. Results: Significant loss of viability resulted from 24-48 h incubation with curcumin. With photodynamic therapy (PDT), even short time incubation (1 h) with curcumin resulted in significantly lower half maximal inhibitory concentration (IC50) (p<0.001, two-way ANOVA). Significant ROS production was observed with PDT and curcumin. Conclusion: Phototherapy strongly enhances the anticancer properties of curcumin in pediatric solid liver tumors in vitro.
引用
收藏
页码：3363 / 3372
页数：10
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