Centrifugal microfluidic system for primary amplification and secondary real-time PCR

被引：69

作者：

Focke, Maximilian ^{[1
]}

Stumpf, Fabian ^{[1
]}

Roth, Guenter ^{[1
,2
]}

Zengerle, Roland ^{[1
,2
,3
]}

von Stetten, Felix ^{[1
,2
]}

机构：

[1] Univ Freiburg, Lab MEMS Applicat, Dept Microsyst Engn IMTEK, D-79110 Freiburg, Germany

[2] HSG IMIT, D-78052 Villingen Schwenningen, Germany

[3] Univ Freiburg, Ctr Biol Signalling Studies Bioss, D-79110 Freiburg, Germany

来源：

LAB ON A CHIP | 2010年 / 10卷 / 23期

关键词：

D O I：

10.1039/c0lc00161a

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Pre-amplification is a basis for numerous polymerase chain reaction (PCR) protocols but bears severe contamination risks due to handling of high-copy DNA samples. Therefore we developed a self-contained centrifugal microfluidic system comprising pre-stored reagents; it enables pre-amplification of specific DNA sequences prior to automated aliquoting and real-time PCR in a modified commercial thermocycler.

引用

页码：3210 / 3212

页数：3

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