Jasmonate-Responsive Transcription Factors NnWRKY70a and NnWRKY70b Positively Regulate Benzylisoquinoline Alkaloid Biosynthesis in Lotus (Nelumbo nucifera)

被引:7
作者
Li, Jing [1 ]
Li, Yi [1 ]
Dang, Mingjing [1 ]
Li, Shang [1 ]
Chen, Simeng [1 ]
Liu, Ruizhen [1 ]
Zhang, Zeyu [1 ]
Li, Guoqian [1 ]
Zhang, Minghua [2 ]
Yang, Dong [2 ]
Yang, Mei [2 ]
Liu, Yanling [2 ]
Tian, Daike [3 ]
Deng, Xianbao [2 ,3 ]
机构
[1] Wuhan Univ Technol, Sch Chem, Chem Engn & Life Sci, Wuhan, Peoples R China
[2] Chinese Acad Sci, Aquat Plant Res Ctr, Wuhan Bot Garden, Wuhan, Peoples R China
[3] Shanghai Chenshan Bot Garden, Shanghai Key Lab Plant Funct Genom & Resources, Shanghai, Peoples R China
来源
FRONTIERS IN PLANT SCIENCE | 2022年 / 13卷
基金
中国国家自然科学基金;
关键词
NnWRKY70; transcriptional regulation; lotus; benzylisoquinoline alkaloid biosynthesis; secondary metabolite; MOLECULAR-CLONING; LEAF SENESCENCE; JAZ PROTEINS; EXPRESSION; FAMILY; IDENTIFICATION; DECARBOXYLASE; SYNTHASE; ENZYME; PLANTS;
D O I
10.3389/fpls.2022.862915
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Lotus (Nelumbo nucifera) is a large aquatic plant that accumulates pharmacologically significant benzylisoquinoline alkaloids (BIAs). However, little is known about their biosynthesis and regulation. Here, we show that the two group III WRKY transcription factors (TFs), NnWRKY70a and NnWRKY70b, positively regulate the BIA biosynthesis in lotus. Both NnWRKY70s are jasmonic acid (JA) responsive, with their expression profiles highly correlated to the BIA concentration and BIA pathway gene expression. A dual-luciferase assay showed that NnWRKY70a could transactivate the NnTYDC promoter, whereas NnWRKY70b could activate promoters of the three BIA structural genes, including NnTYDC, NnCYP80G, and Nn7OMT. In addition, the transient overexpression of NnWRKY70a and NnWRKY70b in lotus petals significantly elevated the BIA alkaloid concentrations. Notably, NnWRKY70b seems to be a stronger BIA biosynthesis regulator, because it dramatically induced more BIA structural gene expressions and BIA accumulation than NnWRKY70a. A yeast two-hybrid assay further revealed that NnWRKY70b physically interacted with NnJAZ1 and two other group III WRKY TFs (NnWRKY53b and NnWRKY70a), suggesting that it may cooperate with the other group III WRKYs to adjust the lotus BIA biosynthesis via the JA-signaling pathway. To illustrate the mechanism underlying NnWRKY70b-mediated BIA regulation in the lotus, a simplified model is proposed. Our study provides useful insights into the regulatory roles of WRKY TFs in the biosynthesis of secondary metabolites.
引用
收藏
页数:16
相关论文
共 55 条
  • [1] WRKY54 and WRKY70 co-operate as negative regulators of leaf senescence in Arabidopsis thaliana
    Besseau, Sebastien
    Li, Jing
    Palva, E. Tapio
    [J]. JOURNAL OF EXPERIMENTAL BOTANY, 2012, 63 (07) : 2667 - 2679
  • [2] The WRKY Transcription Factor Family in Model Plants and Crops
    Chen, Fei
    Hu, Yue
    Vannozzi, Alessandro
    Wu, Kangcheng
    Cai, Hanyang
    Qin, Yuan
    Mullis, Alison
    Lin, Zhenguo
    Zhang, Liangsheng
    [J]. CRITICAL REVIEWS IN PLANT SCIENCES, 2017, 36 (5-6) : 311 - 335
  • [3] Time-course analysis and transcriptomic identification of key response strategies of Nelumbo nucifera to complete submergence
    Deng, Xianbao
    Yang, Dong
    Sun, Heng
    Liu, Juan
    Song, Heyun
    Xiong, Yaqian
    Wang, Yunmeng
    Ma, Junyu
    Zhang, Minghua
    Li, Jing
    Liu, Yanling
    Yang, Mei
    [J]. HORTICULTURE RESEARCH, 2022, 9
  • [4] Investigation of benzylisoquinoline alkaloid biosynthetic pathway and its transcriptional regulation in lotus
    Deng, Xianbao
    Zhao, Li
    Fang, Ting
    Xiong, Yaqian
    Ogutu, Collins
    Yang, Dong
    Vimolmangkang, Sornkanok
    Liu, Yanling
    Han, Yuepeng
    [J]. HORTICULTURE RESEARCH, 2018, 5
  • [5] Analysis of Isoquinoline Alkaloid Composition and Wound-Induced Variation in Nelumbo Using HPLC-MS/MS
    Deng, Xianbao
    Zhu, Lingping
    Fang, Ting
    Vimolmangkang, Sornkanok
    Yang, Dong
    Ogutu, Collins
    Liu, Yanling
    Han, Yuepeng
    [J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2016, 64 (05) : 1130 - 1136
  • [6] The Ubiquitin System and Jasmonate Signaling
    Durand, Astrid Nagels
    Pauwels, Laurens
    Goossens, Alain
    [J]. PLANTS-BASEL, 2016, 5 (01): : 183 - 205
  • [7] MUSCLE: a multiple sequence alignment method with reduced time and space complexity
    Edgar, RC
    [J]. BMC BIOINFORMATICS, 2004, 5 (1) : 1 - 19
  • [8] The WRKY superfamily of plant transcription factors
    Eulgem, T
    Rushton, PJ
    Robatzek, S
    Somssich, IE
    [J]. TRENDS IN PLANT SCIENCE, 2000, 5 (05) : 199 - 206
  • [9] Alkaloid biosynthesis in plants: Biochemistry, cell biology, molecular regulation, and metabolic engineering applications
    Facchini, PJ
    [J]. ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 2001, 52 : 29 - 66
  • [10] FACCHINI PJ, 1994, J BIOL CHEM, V269, P26684